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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/3137

Title: 17α-ethyl-5β-estrane-3α,17β-diol, a biological marker for the abuse of norethandrolone and ethylestrenol in slaughter cattle
Authors: VAN PUYMBROECK, Mark
Kuilman, MEM
Maas, RFM
Witkamp, RF
LEYSSENS, Luc
Van Miert, ASJPAM
Hendriks, L
VANDERZANDE, Dirk
ADRIAENSENS, Peter
JACOBS, Marie-Paule
RAUS, Jef
Issue Date: 1999
Publisher: ELSEVIER SCIENCE BV
Citation: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 728(2). p. 217-232
Abstract: The metabolism of the illegal growth promoter ethylestrenol (EES) was evaluated in bovine liver cells and subcellular fractions of bovine liver preparations. Incubations with bovine microsomal preparations revealed that EES is extensively biotransformed into norethandrolone (NE), another illegal growth promoter. Furthermore, incubations of monolayer cultures of hepatocytes with NE indicated that NE itself is rapidly reduced to 17 alpha-ethyl-5 beta-estrane-3 alpha,17 beta-diol (EED). In vivo tests confirmed that, after administration of either EES or NE, EED is excreted as a major metabolite. Therefore, it was concluded that, both in urine and faeces samples, EED can be used as a biological marker for the illegal use of EES and/or NE. Moreover, by monitoring EED in urine or faeces samples, the detection period after NE administration is significantly prolonged. These findings were further confirmed by three cases of norethandrolone abuse in a routine screening program for forbidden growth promoters. (C) 1999 Elsevier Science B.V. All rights reserved.
Notes: Dr L Willems Inst VZW, Dept Drug & Residue Anal, B-3590 Diepenbeek, Belgium. Limburgs Univ Ctr, Dept SBG, B-3590 Diepenbeek, Belgium. TNO Pharma, NL-3700 AJ Zeist, Netherlands. Univ Utrecht, Dept Vet Pharmacol Pharm & Toxicol, NL-3508 TD Utrecht, Netherlands. Limburgs Univ Ctr, Dept MBW, B-3590 Diepenbeek, Belgium.Van Puymbroeck, M, Dr L Willems Inst VZW, Dept Drug & Residue Anal, Univ Campus,Bldg C, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/3137
DOI: 10.1016/S0378-4347(99)00091-2
ISI #: 000080813300007
ISSN: 0378-4347
Type: Journal Contribution
Validation: ecoom, 2000
Appears in Collections: Research publications

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