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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/3088

Title: Intracellular and luminal pH measurements of Malpighian tubules of the mosquito Aedes aegypti: the effects of cAMP
Authors: Petzel, DH
PIROTTE, Patrick
Issue Date: 1999
Citation: JOURNAL OF INSECT PHYSIOLOGY, 45(11). p. 973-982
Abstract: In order to understand the critical role that hydrogen ions play in fluid secretion in Malpighian tubules, intracellular and luminal pH and K+ measurements were performed in isolated Malpighian tubules of the yellow fever mosquito (Aedes aegypti). The intracellular pH was 7.03+/-0.05 (n=15 Malpighian tubules (MT)) and the luminal pH was 7.19+/-0.09 (n=99 MT) when bathed in saline at a pH of 7.0, The lumen potential is positive, thus net proton secretion into the lumen is active. The intracellular and the luminal K+ concentrations were 75+/-9 mM (n=15) and 102+/-13 mM (n=9 MT) respectively. Cyclic AMP analogues accelerated fluid secretion and at the same time acidified the cell without affecting the luminal pH. Both effects were abolished by an isomer of adenosine-3',5' cyclic monophosphothioate (cAMPS), the Rp-cAMPS, known to inhibit protein kinase A. The results suggest that in the presence of cAMP the properties of the cation/H+ exchanger are affected and that this may be a result of phosphorylation of a Na+/2H+ antiporter located on the apical membrane. (C) 1999 Elsevier Science Ltd. All rights reserved.
Notes: Creighton Univ, Sch Med, Dept Biomed Sci, Omaha, NE 68178 USA. Limburgs Univ Ctr, B-3590 Diepenbeek, Belgium.Petzel, DH, Creighton Univ, Sch Med, Dept Biomed Sci, Omaha, NE 68178 USA.
URI: http://hdl.handle.net/1942/3088
DOI: 10.1016/S0022-1910(99)00076-1
ISI #: 000082195600002
ISSN: 0022-1910
Type: Journal Contribution
Validation: ecoom, 2000
Appears in Collections: Research publications

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