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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/3037

Title: Antitransferrin receptor antibody-RNase fusion protein expressed in the mammary gland of transgenic mice
Authors: Newton, DL
Pollock, D
DiTullio, P
Echelard, Y
Harvey, M
Wilburn, B
Williams, J
Hoogenboom, HR
RAUS, Jef
Meade, H. M.
Rybak, SM
Issue Date: 1999
Publisher: ELSEVIER SCIENCE BV
Citation: JOURNAL OF IMMUNOLOGICAL METHODS, 231(1-2). p. 159-167
Abstract: Antibodies fused to human enzymes offer an alternative to specifically targeting tumors with antibodies linked to plant or bacterial toxins. Since large amounts of these reagents can be administered without eliciting non-specific toxicities, efficient methods of production are needed. The goal of this work was to express a complex immunoenzyme fusion protein (immunotoxin) in the mammary gland of transgenic mice. A chimeric mouse/human antibody directed against the human transferrin receptor (E6) was fused at its CH2 domain to the gene for a human angiogenic ribonuclease, angiogenin (Ang). It was expressed in the mammary gland of mice and secreted into mouse milk. Expression levels in milk were approximately 0.8 g/l. The chimeric protein retained antibody binding activity and protein synthesis inhibitory activity equivalent to that of free Ang. It was specifically cytotoxic to human tumor cells in vitro. (C) 1999 Elsevier Science B.V. All rights reserved.
Notes: NCI, Pharmacol Expt Therapeut Sect, Lab Drug Discovery Res & Dev,Dev Therapeut Program, Div Canc Treatment & Diag,Frederick Canc Res & De, Frederick, MD 21702 USA. NCI, Intramural Res Support Program, SAIC Frederick, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA. Genzyme Transgen, Framingham, MA 01701 USA. Maastricht Univ, Dept Pathol, CESAME, NL-6202 AZ Maastricht, Netherlands. DL Willems Inst, B-3590 Diepenbeek, Belgium.Rybak, SM, NCI, Pharmacol Expt Therapeut Sect, Lab Drug Discovery Res & Dev,Dev Therapeut Program, Div Canc Treatment & Diag,Frederick Canc Res & De, Bldg 567,Room 152, Frederick, MD 21702 USA.
URI: http://hdl.handle.net/1942/3037
DOI: 10.1016/S0022-1759(99)00154-4
ISI #: 000085673800014
ISSN: 0022-1759
Type: Journal Contribution
Validation: ecoom, 2001
Appears in Collections: Research publications

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