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|Title: ||Antitransferrin receptor antibody-RNase fusion protein expressed in the mammary gland of transgenic mice|
|Authors: ||Newton, DL|
Meade, H. M.
|Issue Date: ||1999|
|Publisher: ||ELSEVIER SCIENCE BV|
|Citation: ||JOURNAL OF IMMUNOLOGICAL METHODS, 231(1-2). p. 159-167|
|Abstract: ||Antibodies fused to human enzymes offer an alternative to specifically targeting tumors with antibodies linked to plant or bacterial toxins. Since large amounts of these reagents can be administered without eliciting non-specific toxicities, efficient methods of production are needed. The goal of this work was to express a complex immunoenzyme fusion protein (immunotoxin) in the mammary gland of transgenic mice. A chimeric mouse/human antibody directed against the human transferrin receptor (E6) was fused at its CH2 domain to the gene for a human angiogenic ribonuclease, angiogenin (Ang). It was expressed in the mammary gland of mice and secreted into mouse milk. Expression levels in milk were approximately 0.8 g/l. The chimeric protein retained antibody binding activity and protein synthesis inhibitory activity equivalent to that of free Ang. It was specifically cytotoxic to human tumor cells in vitro. (C) 1999 Elsevier Science B.V. All rights reserved.|
|Notes: ||NCI, Pharmacol Expt Therapeut Sect, Lab Drug Discovery Res & Dev,Dev Therapeut Program, Div Canc Treatment & Diag,Frederick Canc Res & De, Frederick, MD 21702 USA. NCI, Intramural Res Support Program, SAIC Frederick, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA. Genzyme Transgen, Framingham, MA 01701 USA. Maastricht Univ, Dept Pathol, CESAME, NL-6202 AZ Maastricht, Netherlands. DL Willems Inst, B-3590 Diepenbeek, Belgium.Rybak, SM, NCI, Pharmacol Expt Therapeut Sect, Lab Drug Discovery Res & Dev,Dev Therapeut Program, Div Canc Treatment & Diag,Frederick Canc Res & De, Bldg 567,Room 152, Frederick, MD 21702 USA.|
|ISI #: ||000085673800014|
|Type: ||Journal Contribution|
|Validation: ||ecoom, 2001|
|Appears in Collections: ||Research publications|
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