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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/3020

Title: Partial identification of a peptide that stimulates the primary urine production of single isolated Malpighian tubules of the forest ant, Formica polyctena
Authors: Laenen, B
Schoofs, L
Issue Date: 1999
Citation: JOURNAL OF INSECT PHYSIOLOGY, 45(8). p. 743-753
Abstract: A peptide was purified from a 10% trifluoroacetic acid (TFA) head/thorax extract of 300,000 ants with high performance liquid chromatography (HPLC). Fluid secretion assay of single isolated Malpighian tubules was used as a bioassay. The purity of F. polyctena diuretic peptide (FopDP) after a two step HPLC protocol was confirmed by means of mass spectrometry and revealed a molecular mass of 7514 daltons. Due to lack of material, no enzymatic digestion could be performed and the sequence of only the first 25 amino acids could be determined: VPKYENCVSEVLPAGDRQRCVKVTC. A computer search of sequence data banks did not reveal any significant similarity between FopDP and other known insect diuretic peptides. FopDP had no effect on the basolateral membrane potential and depolarised the apical membrane potential of the Malpighian tubule cells. This effect as well as the stimulatory effect on the primary urine formation in the Malpighian tubule of the ant, could be mimicked with A23187, a calcium ionophore, and by thapsigargin, an inhibitor of the endoplasmic reticulum calcium ATPase. FopDP did not stimulate the cAMP content. The results suggest that FopDP uses an increase of intracellular calcium as cellular transduction mechanism. (C) 1999 Elsevier Science Ltd. All rights reserved.
Notes: Limburgs Univ Ctr, Dept MBW Fysiol, B-3590 Diepenbeek, Belgium. Katholieke Univ Leuven, Inst Zool, B-3000 Louvain, Belgium.Van Kerkhove, E, Limburgs Univ Ctr, Dept MBW Fysiol, Univ Campus Gebouw D, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/3020
DOI: 10.1016/S0022-1910(99)00053-0
ISI #: 000081531700006
ISSN: 0022-1910
Type: Journal Contribution
Validation: ecoom, 2000
Appears in Collections: Research publications

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