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Title: Copper affects the enzymes of the ascorbate-glutathione cycle and its related metabolites in the roots of Phaseolus vulgaris
Authors: Gupta, Manisha
Cuypers, Ann
Vangronsveld, Jaco
Clijsters, Herman
Issue Date: 1999
Citation: PHYSIOLOGIA PLANTARUM, 106(3). p. 262-267
Abstract: The involvement of the ascorbate-glutathione cycle in the defence against Cu-induced oxidative stress was studied in the roots of Phaseolus vulgaris is L. cv. Limburgse vroege. All the enzymes of this cycle [ascorbate peroxidase (APOD), EC; monodehydroascorbate reductase (MDHAR), EC; dehydroascorbate reductase (DHAR), EC; glutathione reductase (GR), EC] were increased, and the total ascorbate and glutathione pools rose after a 15 mu M root Cu treatment. In the first hours after the start of the experiment, the accumulation of dehydroascorbate (DHA), formed as a result of a Cu-mediated direct oxidation of ascorbate (AA), was limited by a non-enzymatic reduction using glutathione (GSH) as the reductant. At 24 h, the enzyme capacities of both DHAR and GR were increased to maintain the redox status of the AA and GSH pools. After 72 h of Cu application, the DHAR capacity was inhibited and MDHAR was responsible for maintaining the AA pool in its reduced form. Although the CR capacity was enhanced after 72 h in the treated plants, the GSSG/GSH ratio was increased. This could be due to direct participation of GSH in the detoxification of Cu through reduction and complexation.
Notes: Limburgs Univ Ctr, Ctr Environm Sci, B-3590 Diepenbeek, Belgium.Cuypers, A, Limburgs Univ Ctr, Ctr Environm Sci, Univ Campus, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/3007
DOI: 10.1034/j.1399-3054.1999.106302.x
ISI #: 000082812200002
ISSN: 0031-9317
Type: Journal Contribution
Validation: ecoom, 2000
Appears in Collections: Research publications

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