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Title: Kinetics of phosphate absorption by mycorrhizal and non-mycorrhizal Scots pine seedlings
Authors: VAN TICHELEN, Katia
Issue Date: 2000
Citation: PHYSIOLOGIA PLANTARUM, 110(1). p. 96-103
Abstract: Kinetics of net phosphate (P-i) uptake was measured on intact ectomycorrhizal and non-mycorrhizal Pinus sylvestris seedlings using a semihydroponic cultivation method. The depletion of P-i in a nutrient solution was assessed over a 160-0.2 mu M P-i gradient. Growth of the pine seedlings was P limited and measurements were performed 7 and 9 weeks after inoculation. Three ectomycorrhizal fungi were studied: Paxillus involutus, Suillus bovinus and Thelephora terrestris. P-i uptake was extremely fast in plants colonised by P. involutus. The P-i concentration dropped below 0.2 mu M within 4-5 h. In plants colonised with S. bovinus this occurred in 5-6 h and in plants associated with T. terrestris 8 h were needed to run through the whole concentration range. Non-mycorrhizal plants of similar size and nutrient status decreased P-i to a concentration between 1 and 2 mu M in 18 h. Data were curve fitted to a two-phase Michaelis-Menten equation. The apparent kinetic constants, K-m and V-max, for the high affinity P-i uptake system of the Dine roots could be estimated accurately. V-max of this system was up to 7 times higher in pines associated with P. involutus than in non-mycorrhizal seedlings. The intact extraradical mycelium greatly increased the absorption surface area of the roots (V-max). Non-mycorrhizal plants had a K-m between 7.8 and 16.4 mu M P-i. Plants mycorrhizal with P. involutus had K-m values between 2.4 and 7.2, plants colonised with S. bovinus had a K-m between 5.1 and 12.3, and seedlings associated with T. terrestris had a K-m from 4.6 to 10.1 mu M P-i. All 3 ectomycorrhizal fungi had a strong impact on the P-i absorption capacity of the pine seedlings. The results also demonstrated that there is substantial heterogeneity in kinetic parameters among the different mycorrhizal root systems.
Notes: Limburgs Univ Ctr, Lab Environm Biol, B-3590 Diepenbeek, Belgium.Colpaert, JV, Limburgs Univ Ctr, Lab Environm Biol, Univ Campus, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/2951
ISI #: 000089349700013
ISSN: 0031-9317
Category: A1
Type: Journal Contribution
Validation: ecoom, 2001
Appears in Collections: Research publications

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