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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2903

Title: Encephalomyelitis-associated antimyelin autoreactivity induced by streptococcal exotoxins
Authors: Jorens, PG
VANDER BORGHT, Ann
Ceulemans, B
Van Bever, HP
Bossaert, LL
Ieven, M
Goossens, H
Parizel, PM
Van Dijk, H
RAUS, Jef
STINISSEN, Piet
Issue Date: 2000
Publisher: LIPPINCOTT WILLIAMS & WILKINS
Citation: NEUROLOGY, 54(7). p. 1433-1441
Abstract: Objective: After implicating Streptococcus pyogenes as causing acute disseminated encephalomyelitis (ADEM) in a child, we wanted to prove that in vivo activation of autoreactive T lymphocytes by superantigens of this Streptococcus contributed to the dramatic demyelination. Background: ADEM is a demyelinating disorder of the CNS sharing many similarities with MS. Demyelination in MS is considered to be the result of an autoimmune process mediated by autoreactive T lymphocytes with specificity for myelin antigens. Methods: Phenotypic analysis and proliferation assays on blood monocytes, as well as isolation of myelin basic protein (MBP)-reactive T-cell lines/clones; and TCR repertorium analysis by PCR-ELISA and cytokine production. Results: 1) The blood T-cell receptor (TCR) repertoire was compatible with in vivo expansion induced by S. pyogenes exotoxins, 2) TCR expression analysis indicated clonal expansion of CD8(+) MBP-reactive T cells, suggesting in vivo activation. MBP-reactive T cells showed crossreactivity to S. pyogenes supernatant and exotoxins. 3) Cytokine mRNA quantification of the mononuclear cells revealed a Th2-biased profile. Conclusion: In vivo exposure to S. pyogenes may have induced activation of pathogenic myelin reactive T cells, contributing to the dramatic inflammatory demyelination.
Notes: Univ Antwerp Hosp, Dept Intens Care Med, B-2650 Edegem, Belgium. Univ Antwerp Hosp, Dept Neurol, B-2650 Edegem, Belgium. Univ Antwerp Hosp, Dept Pediat, B-2650 Edegem, Belgium. Univ Antwerp Hosp, Dept Microbiol, B-2650 Edegem, Belgium. Univ Antwerp Hosp, Dept Radiol, B-2650 Edegem, Belgium. Univ Utrecht, Acad Ziekenhuis, Dept Microbiol, Utrecht, Netherlands. Limburgs Univ Ctr, Biomed Onderzoeksinst DWI, Diepenbeek, Belgium.Jorens, PG, Univ Antwerp Hosp, Dept Intens Care Med, Wilrjkstr 10, B-2650 Edegem, Belgium.
URI: http://hdl.handle.net/1942/2903
ISI #: 000086460900008
ISSN: 0028-3878
Category: A1
Type: Journal Contribution
Validation: ecoom, 2001
Appears in Collections: Research publications

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