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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/28728

Title: Transcriptome profiling in blood before and after hepatitis B vaccination shows significant differences in gene expression between responders and non-responders
Authors: Bartholomeus, Esther
De Neuter, Nicolas
Meysman, Pieter
Suls, Arvid
Keersmaekers, Nina
Elias, George
Jansens, Hilde
Hens, Niel
Smits, Evelien
Van Tendeloo, Viggo
Beutels, Philippe
Van Damme, Pierre
Ogunjimi, Benson
Laukens, Kris
Mortier, Geert
Issue Date: 2018
Citation: Vaccine, 36(42), p. 6282-6289
Abstract: Introduction: As the hepatitis B virus is widely spread and responsible for considerable morbidity and mortality, WHO recommends vaccination from infancy to reduce acute infection and chronic carriers. However, current subunit vaccines are not 100% efficacious and leave 5-10% of recipients unprotected. Methods: To evaluate immune responses after Engerix-B vaccination, we determined, using mRNA-sequencing, whole blood early gene expression signatures before, at day 3 and day 7 after the first dose and correlated this with the resulting antibody titer after two vaccine doses. Results: Our results indicate that immune related genes are differentially expressed in responders mostly at day 3 and in non-responders mostly at day 7. The most remarkable difference between responders and non-responders were the differentially expressed genes before vaccination. The granulin precursor gene (GRN) was significantly downregulated in responders while upregulated in non-responders at day 0. Furthermore, absolute granulocytes numbers were significantly higher in non-responders at day 0. Conclusion: The non-responders already showed an activated state of the immune system before vaccination. Furthermore, after vaccination, they exhibited a delayed and partial immune response in comparison to the responders. Our data may indicate that the baseline and untriggered immune system can influence the response upon hepatitis B vaccination. (C) 2018 Elsevier Ltd. All rights reserved.
Notes: [Bartholomeus, Esther; Suls, Arvid; Mortier, Geert] Univ Antwerp, Antwerp Univ Hosp, Dept Med Genet, Prins Boudewijnlaan 43-6, B-2650 Edegem, Belgium. [Bartholomeus, Esther; De Neuter, Nicolas; Meysman, Pieter; Suls, Arvid; Keersmaekers, Nina; Elias, George; Hens, Niel; Smits, Evelien; Van Tendeloo, Viggo; Beutels, Philippe; Van Damme, Pierre; Ogunjimi, Benson; Laukens, Kris; Mortier, Geert] Univ Antwerp, Antwerp Unit Data Anal & Computat Immunol & Seque, AUDACIS, Antwerp, Belgium. [De Neuter, Nicolas; Meysman, Pieter; Laukens, Kris] Univ Antwerp, Dept Math & Comp Sci, Adrem Data Lab, Antwerp, Belgium. [De Neuter, Nicolas; Meysman, Pieter; Laukens, Kris] Univ Antwerp, Biomed Informat Res Network Antwerp Biomina, Antwerp, Belgium. [Keersmaekers, Nina; Hens, Niel; Beutels, Philippe; Ogunjimi, Benson] Univ Antwerp, Vaccine & Infect Dis Inst VAXINFECTIO, CHERMID, Antwerp, Belgium. [Elias, George; Smits, Evelien; Van Tendeloo, Viggo; Ogunjimi, Benson] Univ Antwerp, Vaccine & Infect Dis Inst VAXINFECTIO, LEH, Antwerp, Belgium. [Jansens, Hilde] Antwerp Univ Hosp, Dept Lab Med, Edegem, Belgium. [Hens, Niel] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, Diepenbeek, Belgium. [Hens, Niel; Van Damme, Pierre] Univ Antwerp, Vaccine & Infect Dis Inst VAXINFECTIO, CEV, Antwerp, Belgium. [Smits, Evelien] Antwerp Univ Hosp, Ctr Cell Therapy & Regenerat Med, Edegem, Belgium. [Smits, Evelien] Univ Antwerp, Ctr Oncol Res Antwerp, Antwerp, Belgium. [Ogunjimi, Benson] Antwerp Univ Hosp, Dept Paediat, Edegem, Belgium.
URI: http://hdl.handle.net/1942/28728
DOI: 10.1016/j.vaccine.2018.09.001
ISI #: 000447479200005
ISSN: 0264-410X
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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