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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/28059

Title: Image Correlation Spectroscopy with Second Harmonic Generating Nanoparticles in Suspension and in Cells
Authors: Slenders, Eli
Boye, Hannelore
Urbain, Mathias
Mugnier, Yannick
Sonay, Ali Yasin
Pantazis, Periklis
Bonacina, Luigi
Berghe, Pieter Vanden
vandeVen, Martin
Ameloot, Marcel
Issue Date: 2018
Citation: JOURNAL OF PHYSICAL CHEMISTRY LETTERS, 9(20), p. 6112-6118
Abstract: The absence of photobleaching, blinking, and saturation combined with a high contrast provides unique advantages of higher-harmonic generating nanoparticles over fluorescent probes, allowing for prolonged correlation spectroscopy studies. We apply the coherent intensity fluctuation model to study the mobility of second harmonic generating nanoparticles. A concise protocol is presented for quantifying the diffusion coefficient from a single spectroscopy measurement without the need for separate point-spread-function calibrations. The technique's applicability is illustrated on nominally 56 nm LiNbO3 nanoparticles. We perform label-free raster image correlation spectroscopy imaging in aqueous suspension and spatiotemporal image correlation spectroscopy in A549 human lung carcinoma cells. In good agreement with the expected theoretical result, the measured diffusion coefficient in water at room temperature is (7.5 +/- 0.3) mu m(2)/s. The diffusion coefficient in the cells is more than 10(3) times lower and heterogeneous, with an average of (3.7 +/- 1.5) X 10(-3) mu m(2)/s.
Notes: [Slenders, Eli; Boye, Hannelore; vandeVen, Martin; Ameloot, Marcel] Hasselt Univ, Biomed Res Inst BIOMED, Agoralaan Bldg C, B-3590 Diepenbeek, Belgium. [Urbain, Mathias; Mugnier, Yannick] Univ Savoie Mt Blanc, SYMME, F-74000 Annecy, France. [Sonay, Ali Yasin; Pantazis, Periklis] Swiss Fed Inst Technol, Dept Biosyst Sci & Engn, Mattenstr 26, CH-4058 Basel, Switzerland. [Pantazis, Periklis] Imperial Coll London, Dept Bioengn, South Kensington Campus, London SW7 2AZ, England. [Bonacina, Luigi] Univ Geneva, Dept Appl Phys, Chemin Pinchat 22, CH-1211 Geneva, Switzerland. [Berghe, Pieter Vanden] Univ Leuven, Lab Enter Neurosci LENS, TARGID, Herestr 49, B-3000 Leuven, Belgium.
URI: http://hdl.handle.net/1942/28059
DOI: 10.1021/acs.jpclett.8b02686
ISI #: 000448751700031
ISSN: 1948-7185
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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