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|Title: ||Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy|
|Authors: ||Liu, Jing|
Zanacchi, Francesca Cella
Singh, Santosh K.
Fraire, Juan C.
Samal, Sangram Keshari
De Rycke, Riet
De Smedt, Stefaan C.
|Issue Date: ||2018|
|Publisher: ||CHINESE ACAD SCIENCES, CHANGCHUN INST OPTICS FINE MECHANICS AND PHYSICS|
|Citation: ||LIGHT-SCIENCE & APPLICATIONS, 7 (Art N° 47)|
|Abstract: ||In the replacement of genetic probes, there is increasing interest in labeling living cells with high-quality extrinsic labels, which avoid over-expression artifacts and are available in a wide spectral range. This calls for a broadly applicable technology that can deliver such labels unambiguously to the cytosol of living cells. Here, we demonstrate that nanoparticle-sensitized photoporation can be used to this end as an emerging intracellular delivery technique. We replace the traditionally used gold nanoparticles with graphene nanoparticles as photothermal sensitizers to permeabilize the cell membrane upon laser irradiation. We demonstrate that the enhanced thermal stability of graphene quantum dots allows the formation of multiple vapor nanobubbles upon irradiation with short laser pulses, allowing the delivery of a variety of extrinsic cell labels efficiently and homogeneously into live cells. We demonstrate high-quality time-lapse imaging with confocal, total internal reflection fluorescence (TIRF), and Airyscan super-resolution microscopy. As the entire procedure is readily compatible with fluorescence (super resolution) microscopy, photoporation with graphene quantum dots has the potential to become the long-awaited generic platform for controlled intracellular delivery of fluorescent labels for live-cell imaging.|
|Notes: ||[Liu, Jing; Xiong, Ranhua; Brans, Toon; Fraire, Juan C.; Teirlinck, Eline; Samal, Sangram Keshari] Univ Ghent, Lab Gen Biochem & Phys Pharm, Fac Pharm, B-9000 Ghent, Belgium. [Liu, Jing; Xiong, Ranhua; Brans, Toon; Fraire, Juan C.; Teirlinck, Eline; Samal, Sangram Keshari] Univ Ghent, Ctr Nano & Biophoton, B-9000 Ghent, Belgium. [Lippens, Saskia; Parthoens, Eef] VIB, VIB UGent Ctr Inflammat Res, B-9000 Ghent, Belgium. [Lippens, Saskia; Parthoens, Eef] VIB, VIB Bioimaging Core, B-9000 Ghent, Belgium. [Lippens, Saskia; Parthoens, Eef] Univ Ghent, Dept Biomed Mol Biol, B-9000 Ghent, Belgium. [Zanacchi, Francesca Cella; Magrassi, Raffaella] Ist Italiano Tecnol, Nanophys NAPH, I-16163 Genoa, Italy. [Magrassi, Raffaella] CNR, Biophys Inst IBF, Via Marini 6, I-16149 Genoa, GE, Italy. [Singh, Santosh K.; Kurungot, Sreekumar] CSIR Natl Chem Lab, Phys & Mat Chem Div, Dr Homi Bhabha Rd, Pune 411008, Maharashtra, India. [Singh, Santosh K.; Kurungot, Sreekumar] Acad Sci & Innovat Res, 2 RafiMarg, New Delhi 110001, India. [Szunerits, Sabine; Boukherroub, Rabah] Univ Valenciennes, Univ Lille, CNRS, Cent Lille,ISEN,UMR IEMN 8520, F-59000 Lille, France. [Bove, Hannelore; Ameloot, Marcel] Hasselt Univ, Biomed Res Inst, Agoralaan Bldg C, B-3590 Diepenbeek, Belgium. [Bove, Hannelore; Houthaeve, Gaelle; Braeckmans, Kevin] Katholieke Univ Leuven, Ctr Surface Chem & Catalysis, Celestijnenlaan 200F, B-3001 Leuven, Belgium. [De Rycke, Riet] VIB, Inflammat Res Ctr, Image Core Facil, B-9052 Ghent, Belgium. [De Rycke, Riet] Univ Ghent, Dept Biomed Mol Biol, B-9052 Ghent, Belgium. [De Rycke, Riet] Univ Ghent, Dept Biomed Mol Biol, B-9052 Ghent, Belgium. [De Rycke, Riet] Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium. [Houthaeve, Gaelle] Univ Lille Nord France, Univ Lille 1, Lab Phys Lasers Atomes & Mol, UMR 8523, F-59655 Villeneuve Dascq, France. [De Smedt, Stefaan C.] NFU, Jiangsu Key Lab Biomass Based Green Fuels & Chem, Coll Chem Engn, Nanjing 210037, Jiangsu, Peoples R China. [Braeckmans, Kevin] Univ Lille, UMR 8523, Lab Phys Lasers Atomes & Mol, Villeneuve Dascq, France. [Braeckmans, Kevin] Univ Lille, IEMN, UMR 8520, Villeneuve Dascq, France.|
|ISI #: ||000441267100001|
|Type: ||Journal Contribution|
|Appears in Collections: ||Research publications|
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