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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/28049

Title: Investigating the effect of poly-l-lactic acid nanoparticles carrying hypericin on the flow-biased diffusive motion of HeLa cell organelles
Authors: Penjweini, Rozhin
Deville, Sarah
Maghsoudi, Omid Haji
Notelaers, Kristof
Ethirajan, Anitha
Ameloot, Marcel
Issue Date: 2019
Publisher: WILEY
Citation: JOURNAL OF PHARMACY AND PHARMACOLOGY, 71 (1), p. 104-116
Abstract: Objectives In this study, we investigate in human cervical epithelial HeLa cells the intracellular dynamics and the mutual interaction with the organelles of the poly-l-lactic acid nanoparticles (PLLA NPs) carrying the naturally occurring hydrophobic photosensitizer hypericin. Methods Temporal and spatiotemporal image correlation spectroscopy was used for the assessment of the intracellular diffusion and directed motion of the nanocarriers by tracking the hypericin fluorescence. Using image cross-correlation spectroscopy and specific fluorescent labelling of endosomes, lysosomes and mitochondria, the NPs dynamics in association with the cell organelles was studied. Static colocalization experiments were interpreted according to the Manders' overlap coefficient. Key findings Nanoparticles associate with a small fraction of the whole-organelle population. The organelles moving with NPs exhibit higher directed motion compared to those moving without them. The rate of the directed motion drops substantially after the application of nocodazole. The random component of the organelle motions is not influenced by the NPs. Conclusions Image correlation and cross-correlation spectroscopy are most appropriate to unravel the motion of the PLLA nanocarrier and to demonstrate that the rate of the directed motion of organelles is influenced by their interaction with the nanocarriers. Not all PLLA-hypericin NPs are associated with organelles.
Notes: [Penjweini, Rozhin; Deville, Sarah; Notelaers, Kristof; Ameloot, Marcel] Hasselt Univ, Biomed Res Inst, Agoralaan Bldg C, BE-3590 Diepenbeek, Belgium. [Penjweini, Rozhin] NHLBI, Lab Mol Biophys, NIH, Bldg 10, Bethesda, MD 20892 USA. [Deville, Sarah] Flemish Inst Technol Res, Environm Risk & Hlth Unit, Mol, Belgium. [Maghsoudi, Omid Haji] Temple Univ, Sch Engn, Dept Bioengn, Philadelphia, PA 19122 USA. [Notelaers, Kristof] Maastricht Univ, Div Nanoscopy, Maastricht, Netherlands. [Ethirajan, Anitha] Hasselt Univ, IMO IMOMEC, Inst Mat Res, Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/28049
DOI: 10.1111/jphp.12779
ISI #: 000452534600010
ISSN: 0022-3573
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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