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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/27500

Title: Size-dependent properties of functional PPV-based conjugated polymer nanoparticles for bioimaging
Authors: Peters, Martijn
Seneca, Senne
Hellings, Niels
Junkers, Tanja
Ethirajan, Anitha
Issue Date: 2018
Abstract: Conjugated polymer nanoparticle systems have gained significant momentum in the bioimaging field on account of their biocompatibility and outstanding spectroscopic properties. Recently, new control procedures have spawned custom-built functional poly(p-phenylene vinylene) (PPV). These facilitate the one-pot synthesis of semiconducting polymer NPs with incorporated surface functional groups, an essential feature for advanced biomedical applications. In this work, nanoparticles (NPs) of different sizes are synthesized consisting of the statistical copolymer CPM-co-MDMO-PPV with monomer units 2-(5'-methoxycarbonylpentyloxy)-5-methoxy-1,4-phenylenevinylene (CPM-PPV) and poly(2-methoxy-5-(3',7'-dimethoxyoctyloxy)-1,4-phenylenevinylene) (MDMO-PPV). To monitor potential implications of switching from a commonly used homopolymer to copolymer system, MDMO-PPV NPs were prepared as a control. The versatile combination of the miniemulsion and solvent evaporation method allowed for an easy adaptation of the NP size. Decreasing the diameter of functional PPV-based NPs up to 20 nm did not significantly affect their optical properties nor the biocompatibility of the bioimaging probe, as cell viability never dropped below 90%. The quantum yield and molar extinction coefficient remained stable at values of 1-2% and 10(6) M-1 cm(-1) respectively, indicating an excellent fluorescence brightness. However, a threshold was observed to which the size could be lowered without causing irreversible changes to the system. Cell uptake varied drastically depended on size and material choice, as switching from homo- to copolymer system and lowering the size significantly increased NP uptake, These results clearly demonstrate that adjusting the size of functional PPV-based NPs can be achieved easily to a lower limit of 20 nm without adversely affecting their performance in bioimaging applications.
Notes: [Peters, Martijn; Seneca, Senne; Junkers, Tanja; Ethirajan, Anitha] Hasselt Univ, Inst Mat Res, Martelarenlaan 42, B-3500 Hasselt, Belgium. [Junkers, Tanja; Ethirajan, Anitha] IMEC, Associated Lab IMOMEC, Wetenschapspk 1, B-3590 Diepenbeek, Belgium. [Hellings, Niels] Hasselt Univ, Biomed Res Inst, Immunol Biochem, Agoralaan Bldg C, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/27500
DOI: 10.1016/j.colsurfb.2018.05.055
ISI #: 000440119200058
ISSN: 0927-7765
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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