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|Title: ||T-cell reactivity to multiple myelin antigens in multiple sclerosis patients and healthy controls|
|Authors: ||HELLINGS, Niels|
|Issue Date: ||2001|
|Citation: ||JOURNAL OF NEUROSCIENCE RESEARCH, 63(3). p. 290-302|
|Abstract: ||Myelin proteins, including myelin basic protein (MBP), proteolipid protein (PLP) and myelin oligodendrocyte glycoprotein (MOG) are candidate autoantigens in MS, It is not clear whether MS patients show a predominant reactivity to one or several myelin antigens. We evaluated the IFN-gamma production induced by MBP and MOG and selected MBP-, MOG- and PLP-peptides in MS patients and healthy controls using the IFN-gamma ELISPOT assay. Most MS patients and healthy controls showed a heterogeneous anti-myelin T-cell reactivity, Interestingly in MS patients a positive correlation was found between the anti-MOG and anti-MBP T-cell responses, No myelin peptide was preferentially recognized among the peptides tested (MBP 84-102, 143-168, MOG 1-22, 34-56, 64-86, 74-96, PLP 41-58, 184-199, 190-209). In addition the frequency of IL2R(+) MBP reactive T-cells was significantly increased in blood of MS patients as compared with healthy subjects, indicating that MBP reactive T-cells exist in an in vivo activated state in MS patients. Most of the anti-MBP T-cells were of the Th1-type because reactivity was observed in IFN-gamma but not in IL-4 ELISPOT-assays. Using Thf (IL-12) and Th2 (IL-4) promoting conditions we observed that the cytokine secretion pattern of anti-MBP T-cells still is susceptible to alteration. Our data further indicate that precursor frequency analysis of myelin reactive T-cells by proliferation-based assays may underestimate the true frequency of myelin specific T-cells significantly. (C) 2001 Wiley-Liss, Inc.|
|Notes: ||Limburgs Univ Ctr, Autoimmune Dis Unit, Biomed Onderzoeksinst DWI, B-3590 Diepenbeek, Belgium. Natl MS Centrum, Melsbroek, Belgium. La Trobe Univ, Neuroimmunol Lab, Bundoora, Vic 3083, Australia.Stinissen, P, Limburgs Univ Ctr, Autoimmune Dis Unit, Biomed Onderzoeksinst DWI, Univ Campus,Gebouw A, B-3590 Diepenbeek, Belgium.|
|ISI #: ||000166776600008|
|Type: ||Journal Contribution|
|Validation: ||ecoom, 2002|
|Appears in Collections: ||Research publications|
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