Document Server@UHasselt >
Research >
Research publications >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2707

Title: T-cell reactivity to multiple myelin antigens in multiple sclerosis patients and healthy controls
Authors: HELLINGS, Niels
Baree, M.
Verhoeven, C
D'hooghe, MB
Bernard, C.C.A.
Issue Date: 2001
Publisher: WILEY-LISS
Citation: JOURNAL OF NEUROSCIENCE RESEARCH, 63(3). p. 290-302
Abstract: Myelin proteins, including myelin basic protein (MBP), proteolipid protein (PLP) and myelin oligodendrocyte glycoprotein (MOG) are candidate autoantigens in MS, It is not clear whether MS patients show a predominant reactivity to one or several myelin antigens. We evaluated the IFN-gamma production induced by MBP and MOG and selected MBP-, MOG- and PLP-peptides in MS patients and healthy controls using the IFN-gamma ELISPOT assay. Most MS patients and healthy controls showed a heterogeneous anti-myelin T-cell reactivity, Interestingly in MS patients a positive correlation was found between the anti-MOG and anti-MBP T-cell responses, No myelin peptide was preferentially recognized among the peptides tested (MBP 84-102, 143-168, MOG 1-22, 34-56, 64-86, 74-96, PLP 41-58, 184-199, 190-209). In addition the frequency of IL2R(+) MBP reactive T-cells was significantly increased in blood of MS patients as compared with healthy subjects, indicating that MBP reactive T-cells exist in an in vivo activated state in MS patients. Most of the anti-MBP T-cells were of the Th1-type because reactivity was observed in IFN-gamma but not in IL-4 ELISPOT-assays. Using Thf (IL-12) and Th2 (IL-4) promoting conditions we observed that the cytokine secretion pattern of anti-MBP T-cells still is susceptible to alteration. Our data further indicate that precursor frequency analysis of myelin reactive T-cells by proliferation-based assays may underestimate the true frequency of myelin specific T-cells significantly. (C) 2001 Wiley-Liss, Inc.
Notes: Limburgs Univ Ctr, Autoimmune Dis Unit, Biomed Onderzoeksinst DWI, B-3590 Diepenbeek, Belgium. Natl MS Centrum, Melsbroek, Belgium. La Trobe Univ, Neuroimmunol Lab, Bundoora, Vic 3083, Australia.Stinissen, P, Limburgs Univ Ctr, Autoimmune Dis Unit, Biomed Onderzoeksinst DWI, Univ Campus,Gebouw A, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/2707
DOI: 10.1002/1097-4547(20010201)63:3<290::AID-JNR1023>3.0.CO;2-4
ISI #: 000166776600008
ISSN: 0360-4012
Category: A1
Type: Journal Contribution
Validation: ecoom, 2002
Appears in Collections: Research publications

Files in This Item:

There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.