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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2703

Title: An antidiuretic factor in the forest ant: purification and physiological effects on the Malpighian tubules
Authors: Laenen, B
Nicolson, S
Issue Date: 2001
Citation: JOURNAL OF INSECT PHYSIOLOGY, 47(2). p. 185-193
Abstract: Formica polyctena antidiuretic factor (FopADF) was purified from a 15% trifluoroacetic acid (TFA) extract of the abdomens of 150,000 worker ants. After solid phase extraction of the crude extract and reversed-phase HPLC on two C-18 columns, an antidiuretic factor was isolated. Tested at a concentration of 1.0 ant-equivalents/mul (ant-eq/mul), the factor reversibly inhibited fluid secretion of isolated Malpighian tubules to 29+/-5% (mean+/-SE, n=24) of the control value. The same concentration of FopADF reversibly depolarized both the basolateral membrane potential (V-bl), from -21+/-2 mV to -3+/-1 mV (n=5), and the apical membrane potential (V-ap), from -65+/-5 mV to -20+/-5 mV (n=5). Similar effects on fluid secretion and V-ap were caused by a TFA extract of the haemolymph of ants with non-secreting tubules. Unfortunately, further purification of FopADF on a C-4 column led to a loss of activity in the fluid secretion assay. This is the first time an endogenous antidiuretic factor acting directly on Malpighian tubules has been partially purified and shown to depolarize the tubule cell membranes. (C) 2000 Elsevier Science Ltd. All rights reserved.
Notes: Limburgs Univ Ctr, Dept MBW Fysiol, B-3590 Diepenbeek, Belgium. Univ Cape Town, Dept Zool, ZA-7701 Rondebosch, South Africa.Van Kerkhove, E, Limburgs Univ Ctr, Dept MBW Fysiol, Univ Campus, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/2703
DOI: 10.1016/S0022-1910(00)00104-9
ISI #: 000166325900007
ISSN: 0022-1910
Category: A1
Type: Journal Contribution
Validation: ecoom, 2002
Appears in Collections: Research publications

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