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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2666

Title: Antioxidative enzymes in two wheat cultivars, differently sensitive to drought and subjected to subsymptomatic copper doses
Authors: Sgherri, C
Milone, M.T.A.
Navari-Izzo, F
Issue Date: 2001
Citation: JOURNAL OF PLANT PHYSIOLOGY, 158(11). p. 1439-1447
Abstract: Two wheat cultivars (Triticum durum L. Desf.) differently sensitive to drought (cultivar Ofanto less sensitive than cultivar Adamello) were grown in a controlled environment with subsymptomatic concentrations of Cu. Ethylene emission and enzymes involved in oxygen free radical scavenging were determined. On the basis of a rooting test, cv. Ofanto was shown to be more tolerant to Cu than cv. Adamello. In the leaves, Cu contents were higher in cv. Ofanto than in cv. Adamello, whereas in the roots, Cu level was higher in Adamello, both before and after desorption. During Cu treatment, the increase in ethylene emission was earlier in the more tolerant cultivar, whereas in the leaves, all peroxidase enzymes (ascorbate peroxidase, APOD; guaiacol peroxidase, GPOD; and syringaldazine peroxidase, SPOD) were induced. In both cvs., enzyme activities of SPOD, GPOD, APOD, and superoxide dismutase (SOD) were many-folds higher in the roots than in the leaves. In contrast, catalyse (CAT) activity was higher in the leaves than in the roots. In the control leaves of Adamello, all enzymes showed higher activity in comparison with Ofanto, whereas in the control roots, APOD, GPOD, and SOD were higher in cv. Ofanto than in cv. Adamello. In both cvs., SOD was not involved in the response to Cu excess. CAT activity was not induced in Ofanto leaves and roots under Cu treatment, while an induction was observed in roots and leaves of Adamello subjected to 0.25 mu mol/L Cu. In conclusion, the more tolerant cv. (Ofanto) responds to Cu near threshold concentrations with the induction of peroxidases in the leaves, whereas CAT seems to have a major role in the more sensitive cv. (Adamello).
Notes: Univ Pisa, Dipartimento Chim & Biotecnol Agr, I-56124 Pisa, Italy. Limburgs Univ Ctr, Dept SBG, Lab Environm Biol, B-3590 Diepenbeek, Belgium.Navari-Izzo, F, Univ Pisa, Dipartimento Chim & Biotecnol Agr, Via Borghetto 80, I-56124 Pisa, Italy.
URI: http://hdl.handle.net/1942/2666
DOI: 10.1078/0176-1617-00543
ISI #: 000172442500010
ISSN: 0176-1617
Category: A1
Type: Journal Contribution
Validation: ecoom, 2002
Appears in Collections: Research publications

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