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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/26600

Title: GLI2 promoter hypermethylation in saliva of children with a respiratory allergy
Authors: Langie, Sabine A. S.
Moisse, Matthieu
Szic, Katarzyna Szarc Vel
Van der Plas, Ellen
Koppen, Gudrun
De Prins, Sofie
Louwies, Tijs
Nelen, Vera
Van Camp, Guy
Lambrechts, Diether
Schoeters, Greet
Vanden Berghe, Wim
De Boever, Patrick
Issue Date: 2018
Citation: CLINICAL EPIGENETICS, 10 (Art N° 50)
Abstract: Background: The prevalence of respiratory allergy in children is increasing. Epigenetic DNA methylation changes are plausible underlying molecular mechanisms. Results: Saliva samples collected in substudies of two longitudinal birth cohorts in Belgium (FLEHS1 & FLEHS2) were used to discover and confirm DNA methylation signatures that can differentiate individuals with respiratory allergy from healthy subjects. Genome-wide analysis with Illumina Methylation 450K BeadChips revealed 23 differentially methylated gene regions (DMRs) in saliva from 11y old allergic children (N=26) vs. controls (N=20) in FLEHS1. A subset of 7 DMRs was selected for confirmation by iPLEX MassArray analysis. First, iPLEX analysis was performed in the same 46 FLEHS1 samples for analytical confirmation of the findings obtained during the discovery phase. iPLEX results correlated significantly with the 450K array data (P < 0.0001) and confirmed 4 out of the 7 DMRs. Aiming for additional biological confirmation, the 7 DMRs were analyzed using iPLEX in a substudy of an independent birth cohort (FLEHS2; N=19 cases vs. 20 controls, aged 5 years). One DMR in the GLI2 promoter region showed a consistent statistically significant hypermethylation in individuals with respiratory allergy across the two birth cohorts and technologies. In addition to its involvement in TGF-beta signaling and T-helper differentiation, GLI2 has a regulating role in lung development. Conclusion: GLI2 is considered an interesting candidate DNA methylation marker for respiratory allergy.
Notes: Langie, SAS (reprint author), VITO Sustainable Hlth, Boeretang 200, B-2400 Mol, Belgium. sabine.langie@vito.be
URI: http://hdl.handle.net/1942/26600
DOI: 10.1186/s13148-018-0484-1
ISI #: 000429841500001
ISSN: 1868-7083
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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