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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/26505

Title: Development of a national EUCAST challenge panel for antimicrobial susceptibility testing
Authors: Desmet, S.
Verhaegen, J.
Glupzcynski, Y.
Van Eldere, J.
Melin, P.
Goossens, H.
Pierard, D.
Declercq, P.
Lagrou, K.
Boel, A.
Cartuyvels, R.
Denis, O.
Vandewal, W.
Saegeman, V.
Issue Date: 2016
Abstract: A challenge panel of bacterial strains useful for clinical laboratories to validate their European Committee on Antimicrobial Susceptibility Testing (EUCAST) antimicrobial susceptibility test (AST) system was established. A total of 117 strains, obtained from Belgian Reference Centres (n = 57) and from routine clinical samples (n = 60) was selected based on resistance pattern. These strains were analysed in seven different laboratories by three different automated AST systems (Vitek (n = 2), Phoenix (n = 2) and Microscan (n = 2)) and by disc diffusion from five different manufacturers (Rosco (n = 2), Becton-Dickinson (n = 2), Biomerieux (n = 1), Bio-rad (n = 1) and i2a (n = 1)). To select the challenge panel, selection criteria were set for categorical agreement between the different systems and the number of very major errors, major errors and minor errors. Very major and major errors for at least two antibiotics were observed in 43% of all strains, leading to the exclusion of these strains from the selected panel. In only 10% of all tested strains was there 100% categorical agreement for all antibiotics. Finally, 28 strains (14 Gram-positive and 14 Gram-negative) covering a wide spectrum of resistance mechanisms were selected. Pilot-testing of this challenge panel in 20 laboratories mainly confirmed the results of the validation study. Only six strains withheld for the pilot study could not be used as challenge strain due to an overall (very) major error rate of >5% for a particular antibiotic (n = 5) or for two antibiotics (n = 1). To conclude, this challenge panel should facilitate the implementation and use of EUCAST breakpoints in laboratories. S. Desmet, (C) 2016 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/1942/26505
DOI: 10.1016/j.cmi.2016.05.011
ISI #: WOS:000383824200011
ISSN: 1198-743X
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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