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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/26284

Title: Cell detection by surface imprinted polymers SIPs: A study to unravel the recognition mechanisms
Authors: Yongabi, Derick
Khorshid, Mehran
Losada-Perez, Patricia
Eersels, Kasper
Deschaume, Olivier
D'Haen, Jan
Bartic, Carmen
Hooyberghs, Jef
Thoelen, Ronald
Wubbenhorst, Michael
Wagner, Patrick
Issue Date: 2018
Publisher: ELSEVIER SCIENCE SA
Citation: SENSORS AND ACTUATORS B-CHEMICAL, 255, p. 907-917
Abstract: Previous studies have shown that selective synthetic cell receptors can be produced by cell imprinting on polymer layers. However, knowledge on the fundamental detection mechanisms remains limited. In this article, while using yeast cells (Saccharomyces cerevisiae) as model cells, the factors influencing cellular recognition by surface-imprinted polymers (SIPs) are studied by means of spectroscopic and microscopy techniques and a transducer platform based on interfacial thermal transport, the so-called heat-transfer method (HTM). These analyses indicate that cell imprinting creates selective binding sites on the surface of the SIP layer in the form of binding cavities that match the cells in shape and size. Also, we show that phospholipid moieties are incorporated into the SIP cavities during imprinting, while membrane proteins do not seem to be transferred. More importantly, we demonstrate that the incorporated phospholipids significantly enhance cell adhesion to the SIP, and thus play a significant role in the cell-SIP binding mechanism. Furthermore, the hydrophobicity of the SIP layer was found to be considerably higher when compared with a non-imprinted polymer layer (NIP), an effect that could not be attributed to the presence of cavities on the surface of the SIP layer. Therefore, we suggest that the role of phospholipids in the SIP recognition mechanism is mediated by long range hydrophobic forces. (C) 2017 Elsevier B.V. All rights reserved.
Notes: [Yongabi, Derick; Khorshid, Mehran; Eersels, Kasper; Deschaume, Olivier; Bartic, Carmen; Wubbenhorst, Michael; Wagner, Patrick] KULeuven, Soft Matter Phys & Biophys Sect, Dept Phys & Astron, Celestijnenlaan 200D, B-3001 Leuven, Belgium. [Khorshid, Mehran; Losada-Perez, Patricia; D'Haen, Jan; Thoelen, Ronald] Hasselt Univ, Inst Mat Res IMO, Wetenschapspk 1, B-3590 Diepenbeek, Belgium. [Losada-Perez, Patricia; D'Haen, Jan; Thoelen, Ronald] IMEC Vzw, Div IMOMEC, Wetenschapspk 1, B-3590 Diepenbeek, Belgium. [Eersels, Kasper] Maastricht Univ, Maastricht Sci Programme, NL-6200 MD Maastricht, Netherlands. [Hooyberghs, Jef] VITO, Flemish Inst Technol Res, Boeretang 200, B-2400 Mol, Belgium. [Hooyberghs, Jef] Hasselt Univ, Theoret Phys, Agoralaan,Bldg D, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/26284
DOI: 10.1016/j.snb.2017.08.122
ISI #: 000414151800107
ISSN: 0925-4005
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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