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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/26279

Title: Planarians Customize Their Stem Cell Responses Following Genotoxic Stress as a Function of Exposure Time and Regenerative State
Authors: Stevens, An-Sofie
Wouters, Annelies
Ploem, Jan-Pieter
Pirotte, Nicky
Van Roten, Andromeda
Willems, Maxime
Hellings, Niels
Franken, Carmen
Koppen, Gudrun
Artois, Tom
Plusquin, Michelle
Smeets, Karen
Issue Date: 2018
Citation: TOXICOLOGICAL SCIENCES, 162(1), p. 251-263
Abstract: Aiming to in vivo characterize the responses of pluripotent stem cells and regenerative tissues to carcinogenic stress, we employed the highly regenerative organism Schmidtea mediterranea. Its broad regenerative capacities are attributable to a large pool of pluripotent stem cells, which are considered key players in the lower vulnerability toward chemically induced carcinogenesis observed in regenerative organisms. Schmidtea mediterranea is, therefore, an ideal model to study pluripotent stem cell responses with stem cells residing in their natural environment. Including microenvironmental alterations is important, as the surrounding niche influences the onset of oncogenic events. Both short-(3 days) and long-term (17 days) exposures to the genotoxic carcinogen methyl methanesulfonate (50 mu M) were evaluated during homeostasis and animal regeneration, two situations that render altered cellular niches. In both cases, MMS-induced DNA damage was observed, which provoked a decrease in proliferation on the short term. The outcome of DNA damage responses following long-term exposure differed between homeostatic and regenerating animals. During regeneration, DNA repair systems were more easily activated than in animals in homeostasis, where apoptosis was an important outcome. Knockdown experiments confirmed the importance of DNA repair systems during carcinogenic exposure in regenerating animals as knockdown of rad51 induced a stem cell-depleted phenotype, after regeneration was completed.
Notes: Smeets, K (reprint author), Hasselt Univ, Zool Biodivers & Toxicol, Ctr Environm Sci, Agoralaan,Bldg D, B-3590 Diepenbeek, Belgium, karen.smeets@uhasselt.be
URI: http://hdl.handle.net/1942/26279
DOI: 10.1093/toxsci/kfx247
ISI #: 000427020900025
ISSN: 1096-6080
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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