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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/26085

Title: Children's screen time alters the expression of saliva extracellular miR-222 and miR-146a.
Authors: Vriens, Annette
Provost, Eline B.
Saenen, Nelly D.
De Boever, Patrick
Vrijens, Karen
De Wever, Oliver
Plusquin, Michelle
Nawrot, Tim Steve
Issue Date: 2018
Citation: Scientific Reports, 8(1) (Art N° 8209)
Abstract: An imbalance between energy uptake and energy expenditure is the most important reason for increasing trends in obesity starting from early in life. Extracellular miRNAs are expressed in all bodily fluids and their expression is influenced by a broad range of stimuli. We examined whether screen time, physical activity and BMI are associated with children's salivary extracellular miR-222 and miR-146a expression. In 80 children the extracellular fraction of saliva was obtained by means of differential centrifugation and ultracentrifugation. Expression levels of miR-222 and miR-146a were profiled by qPCR. We studied the association between children's salivary extracellular miRNA expression and screen time, physical activity and BMI using mixed models, while accounting for potential confounders. We found that higher screen time was positively associated with salivary extracellular miR-222 and miR-146a levels. On average, one hour more screen time use per week was associated with a 3.44% higher miR-222 (95% CI: 1.34 to 5.58; p = 0.002) and 1.84% higher miR-146a (95% CI: -0.04 to 3.75; p = 0.055) level in saliva. BMI and physical activity of the child were not significantly associated with either miR-222 or miR-146a. A sedentary behaviour, represented by screen time use in children, is associated with discernible changes in salivary expression of miR-146a and or miR-222. These miRNA targets may emerge attractive candidates to explore the role of these exposures in developmental processes of children's health.
Notes: Nawrot, TS (reprint author), Hasselt Univ, Ctr Environm Sci, Hasselt, Belgium. Leuven Univ, Dept Publ Hlth & Primary Care, Leuven, Belgium. tim.nawrot@uhasselt.be
URI: http://hdl.handle.net/1942/26085
DOI: 10.1038/s41598-018-26351-2
ISI #: 000433289600025
ISSN: 2045-2322
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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