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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2600

Title: A high-performance liquid chromatography/tandem mass spectrometric screening method for eight synthetic corticosteroids in bovine feces and the simultaneous differentiation between dexamethasone and betamethasone
Authors: NOBEN, Jean-Paul
GIELEN, Brigitte
ROYACKERS, Erik
MISSOTTEN, Marleen
Jacobs, A
RAUS, Jef
Issue Date: 2002
Publisher: JOHN WILEY & SONS LTD
Citation: RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 16(16). p. 1590-1594
Abstract: A screening method was developed to monitor the illegal use of synthetic corticosteroids in cattle. Diethyl ether extracts from spiked feces samples were cleaned-up by solid phase extraction followed by semipreparative reversed-phase chromatography (RPC). The fraction containing the corticosteroids was derivatized with ethoxyamine hydrochloride. The corresponding ethoximes were separated using silica-based C18 RPC and analyzed on-line in an ion trap mass spectrometer using atmospheric pressure positive chemical ionization. Ethoxime derivatives of dexamethasone and betamethasone were baseline resolved, allowing for the simultaneous mass spectrometric differentiation of both epimers in bovine feces by conventional non-chiral chromatography. At the lowest level tested (1 mug/kg), corticosteroids (except triamcinolone) could be identified in compliance with the recent European criteria for residue identification. The quantitative performance of the method was best at residue levels greater than or equal to2 mug/kg. Copyright (C) 2002 John Wiley Sons, Ltd.
Notes: Limburgs Univ Ctr, Lab Residue Anal, Inst Biomed Res, B-3590 Diepenbeek, Belgium.Noben, JP, Limburgs Univ Ctr, Lab Residue Anal, Inst Biomed Res, Univ Campus Gebouw C, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/2600
DOI: 10.1002/rcm.752
ISI #: 000177485900013
ISSN: 0951-4198
Category: A1
Type: Journal Contribution
Validation: ecoom, 2003
Appears in Collections: Research publications

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