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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/25854

Title: Abdominal subcutaneous and visceral adipocyte size, lipolysis and inflammation relate to insulin resistance in male obese humans
Authors: Verboven Kenneth
Wouters, Kristiaan
Gaens, Katrien
Hansen, Dominique
Bijnen, Mitchell
Wetzels, Suzan
Stehouwer, Coen D.
Goossens, Gijs H.
Schalkwijk, Casper G.
Blaak, Ellen E.
Jocken, Johan W.
Issue Date: 2018
Citation: Scientific reports (Nature Publishing Group), 8, p. 1-8 (Art N° 4677)
Abstract: Obesity is associated with a disturbed adipose tissue (AT) function characterized by adipocyte hypertrophy, an impaired lipolysis and pro-inflammatory phenotype, which contributes to insulin resistance (IR). We investigated whether AT phenotype in different AT depots of obese individuals with and without type 2 diabetes mellitus (T2DM) is associated with whole-body IR. Subcutaneous (SC) and visceral (V) AT biopsies from 18 lean, 17 obese and 8 obese T2DM men were collected. AT phenotype was characterized by ex vivo measurement of basal and stimulated lipolysis (mature adipocytes), adipocyte size distribution (AT tissue sections) and AT immune cells (flow cytometry). In VAT, mean adipocyte size, CD45+ leukocytes and M1 macrophages were significantly increased in both obese groups compared to lean individuals. In SCAT, despite adipocyte hypertrophy, no significant differences in immune cell populations between groups were found. In SCAT, multiple linear regression analysis showed that none of the AT phenotype markers independently contributed to HOMA-IR while in VAT, mean adipocyte size was significantly related to HOMA-IR. In conclusion, beside adipocyte hypertrophy in VAT, M1 macrophage- or B-cell-mediated inflammation, may contribute to IR, while inflammation in hypertrophic SCAT does not seem to play a major role in IR.
Notes: Verboven, K (reprint author), Maastricht Univ, Med Ctr, NUTRIM Sch Nutr & Translat Res Metab, Dept Human Biol, Maastricht, Netherlands, k.verboven@maastrichtuniversity.nl
URI: http://hdl.handle.net/1942/25854
DOI: 10.1038/s41598-018-22962-x
ISI #: 000427586500001
ISSN: 2045-2322
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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