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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2585

Title: Copper sorption and accumulation by the extraradical mycelium of different Glomus spp. (arbuscular mycorrhizal fungi) isolated from the same polluted soil
Authors: Gonzalez-Chavez, Del Carmen A.
D'Haen, Jan
Vangronsveld, Jaco
Dodd, John C.
Issue Date: 2002
Publisher: KLUWER ACADEMIC PUBL
Citation: PLANT AND SOIL, 240(2). p. 287-297
Abstract: The form and localisation of Cu accumulation in the extraradical mycelium (ERM) of three arbuscular mycorrhizal fungi (AMF), isolated from the same polluted soil contaminated with the Cu and Arsenate, was studied. There were differences in the capacity of the ERM of the three AMF to sorb and accumulate Cu. Glomus caledonium BEG133 had a significantly lower Cu-sorption capacity than Glomus mosseae BEG132 and Glomus claroideum BEG134 isolated from the polluted soil as well as an isolate of G. mosseae BEG25 from a non-polluted soil. This was directly related to the cation exchange capacity (CEC) of the ERM of these fungi. Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM) linked to an energy dispersive X-ray spectrometer (EDAX) gave more detailed information, showing that the ERM of AMF from the polluted soil was able to accumulate Cu in the mucilaginous outer hyphal wall zone, cell wall and inside the hyphal cytoplasm. The EDAX spectra showed that the accumulated Cu was mainly associated with Fe in the mucilaginous outer hyphal wall zone and in the cell wall. Cu was associated with traces of arsenate inside the cytoplasm of the ERM of Glomus mosseae BEG132 but this was not visible inside the ERM of Glomus caledonium BEG133 or Glomus claroideum BEG134. This work suggests that the ERM of AMF is able to sorb and accumulate Cu, but different tolerance mechanisms exist between the three AMF isolated from the same polluted soil providing further evidence for functional diversity within populations of AMF in soils.
Notes: Int Inst Biotechnol, Sittingbourne Res Ctr, Sittingbourne ME9 8HL, Kent, England. Colegio Postgrad, Inst Recursos Nat, Programa Edafol, Area Microbiol Suelos, Montecillo 56230, Mexico, Mexico. Limburgs Univ Ctr, Inst Mat Res, B-3590 Diepenbeek, Belgium.Dodd, JC, Int Inst Biotechnol, Sittingbourne Res Ctr, 1-13 Innovat Bldg 1000, Sittingbourne ME9 8HL, Kent, England.
URI: http://hdl.handle.net/1942/2585
DOI: 10.1023/A:1015794622592
ISI #: 000176005400008
ISSN: 0032-079X
Category: A1
Type: Journal Contribution
Validation: ecoom, 2003
Appears in Collections: Research publications

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