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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2584

Title: Effects of Pb-EDTA and EDTA on oxidative stress reactions and mineral uptake in Phaseolus vulgaris
Authors: Geebelen, Wouter A.
Vangronsveld, Jaco
Driano, Domy C.
Van Poucke, Lucien
Clijsters, Herman
Issue Date: 2002
Citation: PHYSIOLOGIA PLANTARUM, 115(3). p. 377-384
Abstract: Sequestration of Pb by synthetic chelates has been reported to increase bioavailability, uptake, and translocation of this metal in plants. In this work the potential phytotoxic effects of Pb-EDTA were investigated in Phaseolus vulgaris L. cv. Limburgse vroege plants grown on hydroponics. Addition of 50 muM Pb-EDTA to the nutrient solution caused a significant induction of syringaldazine peroxidase (SPOD; EC in roots and primary leaves and guaiacol peroxidase (GPOD; EC in leaves. Addition of 100 muM Pb-EDTA further exacerbated ascorbate peroxidase (APOD; EC, GPOD, dehydroascorbate reductase (DHAR; EC, glutathione reductase (GR; EC and malic enzyme (ME; EC in roots and APOD and ME in primary leaves. Addition of 200 muM Pb-EDTA also induced DHAR in leaves. This induction of peroxidases (SPOD, GPOD, APOD), enzymes of the ascorbate-glutathione cycle (DHAR, GR in roots) and of an NADP(+) reducing enzyme in roots and primary leaves indicates that oxidative stress has been initiated. At 200 muM Pb-EDTA, chlorophyll a and b content in leaves was significantly reduced while visible effects on root morphology and shoot length were observed, while no significant morphological effects were found in the leaves, confirming the sensitive character of the measured enzymes as plant stress indicators. Elevation of the Pb-EDTA concentration in the growth medium significantly reduced the content of Ca, Fe, Mn and Zn taken up by plants, probably due to ion leakage as a result of observed toxicity. Addition of up to 200 muM EDTA increased chelation of divalent cations in nutrient solution resulting in reduced plant uptake of Zn, Cu, Fe and Mn. This did not result in phytotoxicity.
Notes: Limburgs Univ Ctr, Ctr Environm Sci, Lab Inorgan & Phys Chem, B-3590 Diepenbeek, Belgium. Univ Georgia, Savannah River Ecol Lab, Aiken, SC 29802 USA.Vangronsveld, J, Limburgs Univ Ctr, Ctr Environm Sci, Lab Inorgan & Phys Chem, Univ Campus, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/2584
DOI: 10.1034/j.1399-3054.2002.1150307.x
ISI #: 000176442100007
ISSN: 0031-9317
Category: A1
Type: Journal Contribution
Validation: ecoom, 2003
Appears in Collections: Research publications

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