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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/25746

Title: Microbial community structure and activity in trace element-contaminated soils phytomanaged by Gentle Remediation Options (GRO)
Authors: Touceda-Gonzalez, M.
Prieto-Fernandez, A.
Renella, G.
Giagnoni, L.
Sessitsch, A.
Brader, G.
Kumpiene, J.
Dimitriou, I.
Eriksson, J.
Friesl-Hanl, W.
Galazka, R.
Janssen, Jolien
Mench, M.
Mueller, I.
Neu, S.
Puschenreiter, M.
Siebielec, G.
Vangronsveld, Jaco
Kidd, P. S.
Issue Date: 2017
Citation: ENVIRONMENTAL POLLUTION, 231, p. 237-251
Abstract: Gentle remediation options (GRO) are based on the combined use of plants, associated microorganisms and soil amendments, which can potentially restore soil functions and quality. We studied the effects of three GRO (aided-phytostabilisation, in situ stabilisation and phytoexclusion, and aided-phytoextraction) on the soil microbial biomass and respiration, the activities of hydrolase enzymes involved in the biogeochemical cycles of C, N, P, and S, and bacterial community structure of trace element contaminated soils (TECS) from six field trials across Europe. Community structure was studied using denaturing gradient gel electrophoresis (DGGE) fingerprinting of Bacteria, alpha- and beta-Proteobacteria, Actinobacteria and Streptomycetaceae, and sequencing of DGGE bands characteristic of specific treatments. The number of copies of genes involved in ammonia oxidation and denitrification were determined by qPCR. Phytomanagement increased soil microbial biomass at three sites and respiration at the Biogeco site (France). Enzyme activities were consistently higher in treated soils compared to untreated soils at the Biogeco site. At this site, microbial biomass increased from 696 to 2352 mg ATP kg(-1) soil, respiration increased from 7.4 to 40.1 mg C-CO2 kg(-1) soil d(-1), and enzyme activities were 2-11-fold higher in treated soils compared to untreated soil. Phytomanagement induced shifts in the bacterial community structure at both, the total community and functional group levels, and generally increased the number of copies of genes involved in the N cycle (nirK, nirS, nosZ, and amoA). The influence of the main soil physico-chemical properties and trace element availability were assessed and eventual site-specific effects elucidated. Overall, our results demonstrate that phytomanagement of TECS influences soil biological activity in the long term. (C) 2017 Elsevier Ltd. All rights reserved.
Notes: [Touceda-Gonzalez, M.; Prieto-Fernandez, A.; Kidd, P. S.] CSIC, IIAG, Aptdo 122, Santiago De Compostela 15780, Spain. [Renella, G.; Giagnoni, L.] Univ Florence, Dept Agrifood Prod & Environm Sci, Ple Cascine 18, I-50144 Florence, Italy. [Sessitsch, A.; Brader, G.] AIT Austrian Inst Technol GmbH, Ctr Hlth & Bioresources, A-3430 Tulln, Austria. [Kumpiene, J.] Lulea Univ Technol, Waste Sci & Technol, SE-97187 Lulea, Sweden. [Dimitriou, I.] Swedish Univ Agr Sci, Dept Crop Prod Ecol, SE-75007 Uppsala, Sweden. [Eriksson, J.] Swedish Univ Agr Sci, Dept Soil & Environm, SE-75007 Uppsala 17, Sweden. [Friesl-Hanl, W.] AIT Austrian Inst Technol GmbH, Ctr Energy, A-3430 Tulln, Austria. [Galazka, R.; Siebielec, G.] Inst Soil Sci & Plant Cultivat, State Res Inst, Czartoryskich 8, PL-24100 Pulawy, Poland. [Janssen, J.; Vangronsveld, J.] Hasselt Univ, Ctr Environm Sci, 23 Agoralaan Bldg D, B-3590 Diepenbeek, Belgium. [Mench, M.] Univ Bordeaux, BIOGECO, INRA, F-33615 Pessac, France. [Mueller, I.; Neu, S.] Saxon State Off Environm Agr & Geol, Pillnitzer Pl 3, D-01326 Dresden Pillnitz, Germany. [Puschenreiter, M.] Univ Nat Resources & Life Sci Vienna BOKU, Dept Forest & Soil Sci, A-3430 Tulln, Austria.
URI: http://hdl.handle.net/1942/25746
DOI: 10.1016/j.envpol.2017.07.097
ISI #: 000414881000024
ISSN: 0269-7491
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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