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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/25734

Title: A Lytic Providencia rettgeri Virus of Potential Therapeutic Value Is a Deep-Branching Member of the T5virus Genus
Authors: Oliveira, Hugo
Pinto, Graca
Hendrix, Hanne
Noben, Jean-Paul
Gawor, Jan
Kropinski, Andrew M.
Lobocka, Malgorzata
Lavigne, Rob
Azeredo, Joana
Issue Date: 2017
Abstract: Providencia rettgeri is emerging as a new opportunistic pathogen with high antibiotic resistance. The need to find alternative methods to control antibiotic-resistant bacteria and the recent advances in phage therapy motivate the search for new phages able to infect Providencia spp. This study describes the isolation and characterization of an obligatory lytic phage, vB_PreS_PR1 (PR1), with therapeutic potential against drug-resistant P. rettgeri. PR1 is a siphovirus. Its virion DNA size (118,537 bp), transcriptional organization, terminal repeats (10,461 bp), and nicks in the 3'-to-5' strand are similar to those of phage T5. However, sequence similarities of PR1 to phages of the T5virus genus at the DNA and protein levels are limited, suggesting that it belongs to a new species within the Siphoviridae family. PR1 exhibits the ability to kill P. rettgeri antibiotic-resistant strains, is highly specific to the species, and did not present known genomic markers indicating a temperate lifestyle. The lack of homologies between its proteins and proteins of the only other sequenced Providencia prophage, Redjac, suggests that these two phages evolved separately and may target different host proteins. IMPORTANCE The alarming increase in the number of bacteria resistant to antibiotics has been observed worldwide. This is particularly true for Gram-negative bacteria. For certain of their strains, no effective antibiotics are available. Providencia sp. has been a neglected pathogen but is emerging as a multidrug-resistant bacterium. This has revived interest in bacteriophages as alternative therapeutic agents against this bacterium. We describe the morphological, physiological, and genomic characterization of a novel lytic virus, PR1, which is able to kill drug-resistant P. rettgeri clinical isolates. Genomic and phylogenetic analyses indicate that PR1 is a distant relative of T5virus genus representatives. The lack of known virulence-or temperate lifestyle-associated genes in the genome of PR1 makes this phage a potential candidate for therapeutic use. Analysis of its genome also improves our knowledge of the ecology and diversity of T5-like siphoviruses, providing a new link for evolutionary studies of this phage group.
Notes: [Oliveira, Hugo; Pinto, Graca; Azeredo, Joana] Univ Minho, Ctr Biol Engn, Lab Invest Biofilmes Rosario Oliveira, Braga, Portugal. [Hendrix, Hanne; Lavigne, Rob] Katholieke Univ Leuven, Lab Gene Technol, Leuven, Belgium. [Noben, Jean-Paul] Hasselt Univ, Biomed Res Inst, Diepenbeek, Belgium. [Noben, Jean-Paul] Hasselt Univ, Transnat Univ Limburg, Diepenbeek, Belgium. [Gawor, Jan] Polish Acad Sci, Lab DNA Sequencing & Oligonucleotide Synth, Inst Biochem & Biophys, Warsaw, Poland. [Kropinski, Andrew M.] Univ Guelph, Dept Food Sci, Guelph, ON, Canada. [Kropinski, Andrew M.] Univ Guelph, Dept Pathobiol, Guelph, ON, Canada. [Lobocka, Malgorzata] Polish Acad Sci, Inst Biochem & Biophys, Dept Microbial Biochem, Warsaw, Poland. [Lobocka, Malgorzata] Warsaw Univ Life Sci SGGW, Autonomous Dept Microbial Biol, Fac Agr & Biol, Warsaw, Poland.
URI: http://hdl.handle.net/1942/25734
DOI: 10.1128/AEM.01567-17
ISI #: 000415617800014
ISSN: 0099-2240
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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