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Title: Seed Endophyte Microbiome of Crotalaria pumila Unpeeled: Identification of Plant-Beneficial Methylobacteria
Authors: Sánchez-López, Ariadna S.
Pintelon, Isabel
Stevens, Vincent
Imperato, Valeria
Timmermans, Jean-Pierre
González-Chávez, Carmen
Carrillo-González, Rogelio
Van Hamme, Jonathan
Vangronsveld, Jaco
Thijs, Sofie
Issue Date: 2018
Citation: International journal of molecular sciences (Print), 1(19) (Art N° 291)
Abstract: Metal contaminated soils are increasing worldwide. Metal-tolerant plants growing on metalliferous soils are fascinating genetic and microbial resources. Seeds can vertically transmit endophytic microorganisms that can assist next generations to cope with environmental stresses, through yet poorly understood mechanisms. The aims of this study were to identify the core seed endophyte microbiome of the pioneer metallophyte Crotalaria pumila throughout three generations, and to better understand the plant colonisation of the seed endophyte Methylobacterium sp. Cp3. Strain Cp3 was detected in C. pumila seeds across three successive generations and showed the most dominant community member. When inoculated in the soil at the time of flowering, strain Cp3 migrated from soil to seeds. Using confocal microscopy, Cp3-mCherry was demonstrated to colonise the root cortex cells and xylem vessels of the stem under metal stress. Moreover, strain Cp3 showed genetic and in planta potential to promote seed germination and seedling development. We revealed, for the first time, that the seed microbiome of a pioneer plant growing in its natural environment, and the colonisation behaviour of an important plant growth promoting systemic seed endophyte. Future characterization of seed microbiota will lead to a better understanding of their functional contribution and the potential use for seed-fortification applications.
Notes: Thijs, S (reprint author), Hasselt Univ, Ctr Environm Sci, Agoralaan Bldg D, B-3590 Diepenbeek, Belgium, ariadnas@colpos.mx; isabel.pintelon@uantwerpen.be; vincent.stevens@uhasselt.be; valeria.imperato@uhasselt.be; jean-pierre.timmermans@uantwerpen.be; carmeng@colpos.mx; crogelio@colpos.mx; jvanhamme@tru.ca; jaco.vangronsveld@uhasselt.be; sofie.thijs@uhasselt.be
URI: http://hdl.handle.net/1942/25652
DOI: 10.3390/ijms19010291
ISI #: 000424407200284
ISSN: 1661-6596
Category: A2
Type: Journal Contribution
Appears in Collections: Research publications

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