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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/25505

Title: Human Wharton's Jelly-Derived Stem Cells Display a Distinct Immunomodulatory and Proregenerative Transcriptional Signature Compared to Bone Marrow-Derived Stem Cells
Authors: Donders, Raf
Bogie, Jeroen F. J.
Ravanidis, Stylianos
Gervois, Pascal
Vanheusden, Marjan
Marée, Raphaël
Schrynemackers, Marie
Smeets, Hubert J.M.
Pinxteren, Jef
Gijbels, Kristel
Walbers, Sara
Mays, Robert W.
Deans, Robert
Van Den Bosch, Ludo
Stinissen, Piet
Lambrichts, Ivo
Gyselaers, Wilfried
Hellings, Niels
Issue Date: 2018
Citation: Stem cells and development, 27(2), p. 65-84
Abstract: Mesenchymal stromal cells (MSCs) are multipotent stem cells with immunosuppressive and trophic support functions. While MSCs from different sources frequently display a similar appearance in culture, they often show differences in their surface marker and gene expression profiles. Although bone marrow is considered the ‘‘gold standard’’ tissue to isolate classical MSCs (BM-MSC), MSC-like cells are currently also derived from more easily accessible extra-embryonic tissues such as the umbilical cord. In this study, we defined the best way to isolate MSCs from the Wharton’s jelly of the human umbilical cord (WJ-MSC) and assessed the mesenchymal and immunological phenotype of BM-MSC and WJ-MSC. Moreover, the gene expression profile of established WJ-MSC cultures was compared to two different bone marrow-derived stem cell populations (BM-MSC and multipotent adult progenitor cells or MAPC). We observed that explant culturing of Wharton’s jelly matrix is superior to collagenase tissue digestion for obtaining mesenchymal-like cells, with explant isolated cells displaying increased expansion potential. While being phenotypically similar to adult MSCs, WJ-MSC show a different gene expression profile. Gene ontology analysis revealed that genes associated with cell adhesion, proliferation, and immune system functioning are enriched in WJ-MSC. In vivo transplantation confirms their immune modulatory effect on T cells, similar to BMMSC and MAPC. Furthermore, WJ-MSC intrinsically overexpress genes involved in neurotrophic support and their secretome induces neuronal maturation of SH-SY5Y neuroblastoma cells to a greater extent than BM-MSC. This signature makes WJ-MSC an attractive candidate for cell-based therapy in neurodegenerative and immune-mediated central nervous system disorders such as multiple sclerosis, Parkinson’s disease, or amyotrophic lateral sclerosis.
URI: http://hdl.handle.net/1942/25505
DOI: 10.1089/scd.2017.0029
ISI #: 000418565900001
ISSN: 1547-3287
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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