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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/25491

Title: Calcaneal Tendon Collagen Fiber Morphometry and Aging
Authors: Hadraba, Daniel
Janacek, Jiri
Filova, Eva
Lopot, Frantisek
Paesen, Rik
Fanta, Ondrej
Jarman, Anneliese
Necas, Alois
Ameloot, Marcel
Jelen, Karel
Issue Date: 2017
Publisher: CAMBRIDGE UNIV PRESS
Citation: MICROSCOPY AND MICROANALYSIS, 23(5), p. 1040-1047
Abstract: Fibrillar collagen in tendons and its natural development in rabbits are discussed in this paper. Achilles tendons from newborn (similar to 7 days) to elderly (similar to 38 months) rabbits were monitored in intact (n(tendons) = 24) and microtome sectioned (ntendons = 11) states with label-free second harmonic generation microscopy. After sectioning, the collagen fiber pattern was irregular for the younger animals and remained oriented parallel to the load axis of the tendon for the older animals. In contrast, the collagen fiber pattern in the intact samples followed the load axis for all the age groups. However, there was a significant difference in the tendon crimp pattern appearance between the age groups. The crimp amplitude (A) and wavelength (Lambda) started at very low values (A = 2.0 +/- 0.6 mu m, Lambda = 19 +/- 4 mu m) for the newborn animals. Both parameters increased for the sexually mature animals (>5 months old). When the animals were fully mature the amplitude decreased but the wavelength kept increasing. The results revealed that the microtome sectioning artifacts depend on the age of animals and that the collagen crimp pattern reflects the physical growth and development.
Notes: [Hadraba, Daniel; Janacek, Jiri; Jelen, Karel] Czech Acad Sci, Inst Physiol, Dept Biomath, Videnska 1083, Prague 14220 4, Czech Republic. [Hadraba, Daniel; Lopot, Frantisek; Fanta, Ondrej] Charles Univ Prague, Fac Phys Educ & Sport, Dept Anat & Biomech, Jose Martiho 31, Prague 16200 6, Czech Republic. [Hadraba, Daniel; Paesen, Rik; Ameloot, Marcel] Hasselt Univ, Biomed Res Inst, Dept Biophys, Agoralaan Bldg C, B-3590 Diepenbeek, Belgium. [Filova, Eva] Czech Acad Sci, Inst Expt Med, Dept Tissue Engn, Videnska 1083, Prague 14220 4, Czech Republic. [Jarman, Anneliese] Kings Coll London, Dept Tissue Engn & Biophoton, Guys Campus, London SE1 9RT, England. [Necas, Alois] Univ Vet & Pharmaceut Sci Brno, Fac Vet Med, Palackeho Tr 1-3, Brno 61242, Czech Republic.
URI: http://hdl.handle.net/1942/25491
DOI: 10.1017/S1431927617012569
ISI #: 000412127300017
ISSN: 1431-9276
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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