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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/24983

Title: Regulatory networks underlying mycorrhizal development delineated by genome-wide expression profiling and functional analysis of the transcription factor repertoire of the plant symbiotic fungus Laccaria bicolor
Authors: Daguerre, Y.
Levati, E.
Ruytinx, Joske
Tisserant, E.
Morin, E.
Kohler, A.
Montanini, B.
Ottonello, S.
Brun, A.
Veneault-Fourrey, C.
Martin, F.
Issue Date: 2017
Citation: BMC GENOMICS, 18, p. 1-23 (Art N° 737)
Abstract: Background: Ectomycorrhizal (ECM) fungi develop a mutualistic symbiotic interaction with the roots of their host plants. During this process, they undergo a series of developmental transitions from the running hyphae in the rhizosphere to the coenocytic hyphae forming finger-like structures within the root apoplastic space. These transitions, which involve profound, symbiosis-associated metabolic changes, also entail a substantial transcriptome reprogramming with coordinated waves of differentially expressed genes. To date, little is known about the key transcriptional regulators driving these changes, and the aim of the present study was to delineate and functionally characterize the transcription factor (TF) repertoire of the model ECM fungus Laccaria bicolor. Results: We curated the L. bicolor gene models coding for transcription factors and assessed their expression and regulation in Poplar and Douglas fir ectomycorrhizae. We identified 285 TFs, 191 of which share a significant similarity with known transcriptional regulators. Expression profiling of the corresponding transcripts identified TF-encoding fungal genes differentially expressed in the ECM root tips of both host plants. The L. bicolor core set of differentially expressed TFs consists of 12 and 22 genes that are, respectively, upregulated and downregulated in symbiotic tissues. These TFs resemble known fungal regulators involved in the control of fungal invasive growth, fungal cell wall integrity, carbon and nitrogen metabolism, invasive stress response and fruiting-body development. However, this core set of mycorrhiza-regulated TFs seems to be characteristic of L. bicolor and our data suggest that each mycorrhizal fungus has evolved its own set of ECM development regulators. A subset of the above TFs was functionally validated with the use of a heterologous, transcription activation assay in yeast, which also allowed the identification of previously unknown, transcriptionally active yet secreted polypeptides designated as Secreted Transcriptional Activator Proteins (STAPs). Conclusions: Transcriptional regulators required for ECM symbiosis development in L. bicolor have been uncovered and classified through genome-wide analysis. This study also identifies the STAPs as a new class of potential ECM effectors, highly expressed in mycorrhizae, which may be involved in the control of the symbiotic root transcriptome.
Notes: [Daguerre, Y.; Ruytinx, J.; Tisserant, E.; Morin, E.; Kohler, A.; Brun, A.; Veneault-Fourrey, C.; Martin, F.] Univ Lorraine, INRA, UMR 1136, Interact Arbres Microorganismes,Lab Excellence AR, F-54280 Champenoux, France. [Daguerre, Y.; Ruytinx, J.; Tisserant, E.; Morin, E.; Kohler, A.; Brun, A.; Veneault-Fourrey, C.; Martin, F.] Univ Lorraine, UMR 1136, INRA, Interact Arbres Microorganismes,Lab Excellence AR, F-54500 Nancy, France. [Levati, E.; Montanini, B.; Ottonello, S.] Univ Parma, Dipartimento Sci Chim Vita & Sostenibilita Ambien, Parco Area Sci 23-A, I-43124 Parma, Italy. [Daguerre, Y.] Swedish Univ Agr Sci, Umea Plant Sci Ctr, Dept Forest Genet & Plant Physiol, S-90183 Umea, Sweden. [Ruytinx, J.] Hasselt Univ, Ctr Environm Sci, Agoralaan Bldg D, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/24983
DOI: 10.1186/s12864-017-4114-7
ISI #: 000410996700005
ISSN: 1471-2164
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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