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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/24977

Title: ACCERBATIN, a small molecule at the intersection of auxin and reactive oxygen species homeostasis with herbicidal properties
Authors: Hu, Yuming
Depaepe, Thomas
Smet, Dajo
Hoyerova, Klara
Klima, Petr
Cuypers, Ann
Cutler, Sean
Buyst, Dieter
Morreel, Kris
Boerjan, Wout
Martins, Jose
Petrasek, Jan
Vandenbussche, Filip
Van der Straeten, Dominique
Issue Date: 2017
Citation: JOURNAL OF EXPERIMENTAL BOTANY, 68(15), p. 4185-4203
Abstract: The volatile two-carbon hormone ethylene acts in concert with an array of signals to affect etiolated seedling development. From a chemical screen, we isolated a quinoline carboxamide designated ACCERBATIN (AEX) that exacerbates the 1-aminocyclopropane-1-carboxylic acid-induced triple response, typical for ethylene-treated seedlings in darkness. Phenotypic analyses revealed distinct AEX effects including inhibition of root hair development and shortening of the root meristem. Mutant analysis and reporter studies further suggested that AEX most probably acts in parallel to ethylene signaling. We demonstrated that AEX functions at the intersection of auxin metabolism and reactive oxygen species (ROS) homeostasis. AEX inhibited auxin efflux in BY-2 cells and promoted indole-3-acetic acid (IAA) oxidation in the shoot apical meristem and cotyledons of etiolated seedlings. Gene expression studies and superoxide/hydrogen peroxide staining further revealed that the disrupted auxin homeostasis was accompanied by oxidative stress. Interestingly, in light conditions, AEX exhibited properties reminiscent of the quinoline carboxylate-type auxin-like herbicides. We propose that AEX interferes with auxin transport from its major biosynthesis sites, either as a direct consequence of poor basipetal transport from the shoot meristematic region, or indirectly, through excessive IAA oxidation and ROS accumulation. Further investigation of AEX can provide new insights into the mechanisms connecting auxin and ROS homeostasis in plant development and provide useful tools to study auxin-type herbicides.
Notes: [Hu, Yuming; Depaepe, Thomas; Smet, Dajo; Vandenbussche, Filip; Van der Straeten, Dominique] Univ Ghent, Fac Sci, Dept Biol, Lab Funct Plant Biol, KL Ledeganckstr 35, B-9000 Ghent, Belgium. [Klima, Petr; Petrasek, Jan] Inst Expt Bot ASCR, Prague 16500 6, Czech Republic. [Cuypers, Ann] Hasselt Univ, Ctr Environm Sci, Agoralaan Bldg D, B-3590 Diepenbeek, Belgium. [Cutler, Sean] Univ Calif Riverside, Inst Integrat Genome Biol, Dept Bot & Plant Sci, Riverside, CA 92521 USA. [Buyst, Dieter; Martins, Jose] Dept Organ Chem, NMR & Struct Anal, Krijgslaan 281, B-9000 Ghent, Belgium. [Morreel, Kris; Boerjan, Wout] VIB Flanders Inst Biotechnol, Dept Plant Syst Biol, Technol Pk 927, B-9052 Ghent, Belgium.
URI: http://hdl.handle.net/1942/24977
DOI: 10.1093/jxb/erx242
ISI #: 000410245700017
ISSN: 0022-0957
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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