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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/24965

Title: Identification and Quantification of Celery Allergens Using Fiber Optic Surface Plasmon Resonance PCR
Authors: Daems, Devin
Peeters, Bernd
Delport, Filip
Remans, Tony
Lammertyn, Jeroen
Spasic, Dragana
Issue Date: 2017
Publisher: MDPI AG
Citation: SENSORS, 17(8), p. 1-10 (Art N° 1754)
Abstract: Accurate identification and quantification of allergens is key in healthcare, biotechnology and food quality and safety. Celery (Apium graveolens) is one of the most important elicitors of food allergic reactions in Europe. Currently, the golden standards to identify, quantify and discriminate celery in a biological sample are immunoassays and two-step molecular detection assays in which quantitative PCR (qPCR) is followed by a high-resolution melting analysis (HRM). In order to provide a DNA-based, rapid and simple detection method suitable for one-step quantification, a fiber optic PCR melting assay (FO-PCR-MA) was developed to determine different concentrations of celery DNA (1 pM-0.1 fM). The presented method is based on the hybridization and melting of DNA-coated gold nanoparticles to the FO sensor surface in the presence of the target gene (mannitol dehydrogenase, Mtd). The concept was not only able to reveal the presence of celery DNA, but also allowed for the cycle-to-cycle quantification of the target sequence through melting analysis. Furthermore, the developed bioassay was benchmarked against qPCR followed by HRM, showing excellent agreement (R-2 = 0.96). In conclusion, this innovative and sensitive diagnostic test could further improve food quality control and thus have a large impact on allergen induced healthcare problems.
Notes: [Daems, Devin; Peeters, Bernd; Delport, Filip; Lammertyn, Jeroen; Spasic, Dragana] Univ Leuven, KU Leuven, BIOSYST MeBioS, Willem de Croylaan 42, B-3001 Leuven, Belgium. [Delport, Filip; Lammertyn, Jeroen] FOx Diagnost NV, Veldstr 120, B-9140 Temse, Belgium. [Remans, Tony] UHasselt Hasselt Univ, Environm Biol, Agoralaan Gebouw D, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/24965
DOI: 10.3390/s17081754
ISI #: 000408576900057
ISSN: 1424-8220
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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