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|Title: ||Apoptosis in nociceptive desensitized animals after application of capsaicin|
|Authors: ||Krage, T|
|Issue Date: ||2002|
|Publisher: ||INT AMER ASSOC DENTAL RESEARCHI|
|Citation: ||JOURNAL OF DENTAL RESEARCH, 81. p. A62-A62|
|Abstract: ||Objectives: Capsaicin, the pungent ingredient in chili peppers, has the ability systemically to selectively desensitize nociceptive fibers. Furthermore, it is known that the dentinal development in these nociceptive desensitized animals is drastically altered. The aim of this study was to better understand the physiological effects of the neonatal systemic application of capsaicin by investigating the apoptosis, or programmed cell death, in their pulpal tissue. Methods: 20 rats were given a dose of 40mg/kg body weight capsaicin on the first post-natal day. A 50% death rate was observed immediately after capsaicin application. An equal sum of untreated rats served as the control group. All animals were intravitally perfused with a 4% paraformaldehyde and 0.2% picric acid solution at 150 days of age and jaws were dissected. The molars of animals from both study groups were cryosectioned at 20µm and the In Situ Cell Death Detection, POD (Roche) TUNEL reaction was applied to detect DNA strand breakage. Results: While a general observation of apoptosis was observed in both groups, the capsaicin-treated group showed higher numbers of TUNEL-positive cells in the odonblast layer. This finding is a possible explanation for defects seen in dentinal development of capsaicin-treated animals. Conclusion: We may conclude that the systemic neonatal application with capsaicin is in relation with an increase in apoptosis of odontoblasts, thus possibly causing an alteration in the dentin production in these nociceptive desensitized animals.|
|Notes: ||Univ Dusseldorf, D-4000 Dusseldorf, Germany. Limburgs Univ Centrum, Diepenbeek, Belgium.|
|ISI #: ||000176024700286|
|Type: ||Journal Contribution|
|Appears in Collections: ||Research publications|
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