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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/24886

Title: Whole-Genome Saliva and Blood DNA Methylation Profiling in Individuals with a Respiratory Allergy
Authors: Langie, Sabine A. S.
Szic, Katarzyna Szarc vel
Declerck, Ken
Traen, Sophie
Koppen, Gudrun
Van Camp, Guy
Schoeters, Greet
Vanden Berghe, Wim
De Boever, Patrick
Issue Date: 2016
Citation: PLoS One, 11(3), p. 1-17 (Art N° e0183088)
Abstract: The etiology of respiratory allergies (RA) can be partly explained by DNA methylation changes caused by adverse environmental and lifestyle factors experienced early in life. Longitudinal, prospective studies can aid in the unravelment of the epigenetic mechanisms involved in the disease development. High compliance rates can be expected in these studies when data is collected using non-invasive and convenient procedures. Saliva is an attractive biofluid to analyze changes in DNA methylation patterns. We investigated in a pilot study the differential methylation in saliva of RA (n = 5) compared to healthy controls (n = 5) using the Illumina Methylation 450K BeadChip platform. We evaluated the results against the results obtained in mononuclear blood cells from the same individuals. Differences in methylation patterns from saliva and mononuclear blood cells were clearly distinguishable (P-Adj<0.001 and vertical bar Delta beta vertical bar 0.2), though the methylation status of about 96% of the cg-sites was comparable between peripheral blood mononuclear cells and saliva. When comparing RA cases with healthy controls, the number of differentially methylated sites (DMS) in saliva and blood were 485 and 437 (P<0.05 and vertical bar Delta beta vertical bar>0.1), respectively, of which 216 were in common. The methylation levels of these sites were significantly correlated between blood and saliva. The absolute levels of methylation in blood and saliva were confirmed for 3 selected DMS in the PM20D1, STK32C, and FGFR2 genes using pyrosequencing analysis. The differential methylation could only be confirmed for DMS in PM20D1 and STK32C genes in saliva. We show that saliva can be used for genome-wide methylation analysis and that it is possible to identify DMS when comparing RA cases and healthy controls. The results were replicated in blood cells of the same individuals and confirmed by pyrosequencing analysis. This study provides proof-of-concept for the applicability of saliva-based whole-genome methylation analysis in the field of respiratory allergy.
URI: http://hdl.handle.net/1942/24886
DOI: 10.1371/journal.pone.0151109
ISI #: 000372694700021
ISSN: 1932-6203
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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