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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2462

Title: K+ transport in Malpighian tubules of Tenebrio molitor L.: a study of electrochemical gradients and basal K+ uptake mechanisms
Authors: WIEHART, Ursula
Nicolson, SW
VAN KERKHOVE, Emmy
Issue Date: 2003
Publisher: COMPANY OF BIOLOGISTS LTD
Citation: JOURNAL OF EXPERIMENTAL BIOLOGY, 206(6). p. 949-957
Abstract: Malpighian tubules of the mealworm Tenebrio molitor were isolated for intracellular measurement of basolateral (V-bl) and, indirectly, apical (V-ap) membrane potentials. In control Ringer (50 mmol 1(-1) K+, 140 mmol 1(-1) Na+), V-bl was 24mV, cell negative, and V-ap was 48mV, cell negative with reference to the lumen. Ion substitution experiments involving K+ and Na+ indicated that both V-bl and V-ap were sensitive to the bathing K+ concentration, with the change in Yap being 60-77% that of Y-bl. A 10-fold drop in bath [K+] irreversibly decreased fluid secretion rates from 6.38+/-0.95 nl min(-1) (mean +/- S.E.M.) to 1.48 +/- 0.52 nl min-1 (N=8). In the presence of 6 mmol 1(-1) Ba2+, a blocker of basal K+ channels, fluid secretion rates reversibly decreased and the hyperpolarization of both V-bl and Yap seen in 50 mmol 1(-1) and 140 mmol 1(-1) K+ indicated a favourable electrochemical gradient for basal K+ entry. In 5 mmol 1(-1) K+, Ba2+ induced two different responses: V-bl either hyperpolarized by approximately 10 mV or depolarised by approximately 14 mV, according to the electrochemical gradient for K+, which was either inward or outward in low bath [K+]. Rubidium, a `permeant' potassium substitute, caused a hyperpolarization of V-bl, indicating the specificity of K+ channels found in Tenebrio tubule cells. Other possible K+ uptake mechanisms located in the basolateral membrane were investigated. Blocking of the putative electroneutral Na+/K+/2Cl(-) cotransporter by 10 mumol 1(-1) bumetanide reversibly decreased fluid secretion rates, with no detectable change in membrane potentials. Ouabair (1 mmol 1(-1)), an Na+/K+-ATPase inhibitor, irreversibly decreased fluid secretion rates but had no effect on electrical potential differences either in the absence or presence of Ba2+. The results implicate K+ channels, the Na+/K+/2Cl(-) cotransporter and the Na+/K+-ATPase in basal K+ and fluid transport of Tenebrio tubule cells.
Notes: Limburgs Univ Ctr, Biomed CMK, Physiol Lab, B-3590 Diepenbeek, Belgium. Univ Pretoria, Dept Zool & Entomol, ZA-0002 Pretoria, South Africa.Van Kerkhove, E, Limburgs Univ Ctr, Biomed CMK, Physiol Lab, Univ Campus, B-3590 Diepenbeek, Belgium.emmy.vankerkhove@luc.ac.be
URI: http://hdl.handle.net/1942/2462
DOI: 10.1242/jeb.00200
ISI #: 000181805500002
ISSN: 0022-0949
Category: A1
Type: Journal Contribution
Validation: ecoom, 2004
Appears in Collections: Research publications

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