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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2457

Title: Body extracts from the forest ant and honeybee: chromatographic comparison of factors affecting the ant Malpighian tubule
Authors: Laenen, B
WIEHART, Ursula
DE DECKER, Nadine
STEELS, Paul
VAN KERKHOVE, Emmy
Issue Date: 2003
Publisher: BLACKWELL PUBLISHING LTD
Citation: PHYSIOLOGICAL ENTOMOLOGY, 28(4). p. 306-314
Abstract: Crude trifluoroacetic acid extracts were prepared from different body parts of the forest ant, Formica polyctena Foerster, Hymenoptera, Formicidae, and the honeybee, Apis mellifera carnica Pollmann, Hymenoptera, Apidae. Extracts were prepurified by means of solid phase extraction over reversed-phase cartridges. The effects of the resulting fractions on fluid secretion rates were tested on single preparations of ant Malpighian tubules. Diuretic and antidiuretic factors were shown to be present. High-performance liquid chromatography allowed for the identification of a diuretic factor from the bee-head extract which had a similar elution profile to a previously purified diuretic peptide from ant-head extracts. An antidiuretic factor was also identified from the bee abdominal extract. Similar to an antidiuretic peptide, which was purified from ant abdominal extracts, this antidiuretic factor inhibited fluid secretion and depolarized the transepithelial potential of ant Malpighian tubules.
Notes: Limburgs Univ Ctr, CMK Biomed, Physiol Lab, B-3590 Diepenbeek, Belgium. Univ Pretoria, Dept Zool & Entomol, ZA-0002 Pretoria, South Africa.Van Kerkhove, E, Limburgs Univ Ctr, CMK Biomed, Physiol Lab, Univ Campus, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/2457
DOI: 10.1111/j.1365-3032.2003.00349.x
ISI #: 000187256800009
ISSN: 0307-6962
Category: A1
Type: Journal Contribution
Validation: ecoom, 2004
Appears in Collections: Research publications

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