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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2456

Title: Skeletal muscle repair by adult human mesenchymal stem cells from synovial membrane
Authors: De Bari, C
Dell'Accio, F
VANDENABEELE, Frank
Vermeesch, JR
Raymackcrs, JM
Luyten, FP
Issue Date: 2003
Publisher: ROCKEFELLER UNIV PRESS
Citation: JOURNAL OF CELL BIOLOGY, 160(6). p. 909-918
Abstract: W e have demonstrated previously that adult human synovial membrane-derived mesenchymal stem cells (hSM-MSCs) have myogenic potential in vitro (De Bari, C., F Dell'Accio, P. Tylzanowski, and F.P. Luyten. 2001. Arthritis Rheum. 44:192 8-1942). In the present study, we have characterized their myogenic differentiation in a nude mouse model of skeletal muscle regeneration and provide proof of principle of their potential use for muscle repair in the mdx mouse model of Duchenne muscular dystrophy. When implanted into regenerating nude mouse muscle, hSM-MSCs contributed to myofibers and to long term persisting functional satellite cells. No nuclear fusion hybrids were observed between donor human cells and host mouse muscle cells. Myogenic differentiation proceeded through a molecular cascade resembling embryonic muscle development. Differentiation was sensitive to environmental cues, since hSM-MSCs injected into the bloodstream engrafted in several tissues, but acquired the muscle phenotype only within skeletal muscle. When administered into dystrophic muscles of immunosuppressed mdx mice, hSM-MSCs restored sarcolemmal expression of dystrophin, reduced central nucleation, and rescued the expression of mouse mechano growth factor.
Notes: Katholieke Univ Leuven, Univ Hosp, Dept Rheumatol, Lab Skeletal Dev & Joint Disorders, B-3000 Louvain, Belgium. Limburgs Univ Ctr, DWI, Inst Biomed Res, Histol Lab, B-3590 Diepenbeek, Belgium. Katholieke Univ Leuven, Univ Hosp, Ctr Human Genet, B-3000 Louvain, Belgium. Univ Catholique Louvain, Dept Physiol & Pharmacol, B-1200 Brussels, Belgium.Luyten, FP, Katholieke Univ Leuven, Dept Rheumatol, Lab Skeletal Dev & Joint Disorders, Herestr 49, B-3000 Louvain, Belgium.frank.luyten@uz.kuleuven.ac.be
URI: http://hdl.handle.net/1942/2456
DOI: 10.1083/jcb.200212064
ISI #: 000181677500011
ISSN: 0021-9525
Category: A1
Type: Journal Contribution
Validation: ecoom, 2004
Appears in Collections: Research publications

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