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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2451

Title: Characterization of Na+ currents in isolated dorsal unpaired median neurons of Locusta migratoria and effect of the alpha-like scorpion toxin BmK M1
Authors: BRONE, Bert
Tytgat, J
Wang, DC
Issue Date: 2003
Citation: JOURNAL OF INSECT PHYSIOLOGY, 49(2). p. 171-182
Abstract: A primary cell culture was developed for efferent dorsal unpaired median (DUM) neurons of the locust. The isolated somata were able to generate Tetrodotoxin (TTX)-sensitive action potentials in vitro. The a-like scorpion toxin BmK M1, from the Asian scorpion Buthus martensi Karsch, prolonged the duration of the action potential up to 50 times. To investigate the mechanism of action of BmK M1, the TTX-sensitive voltage gated Na+ currents were studied in detail using the whole cell patch clamp technique. BmK M1 slowed down and partially inhibited the inactivation of the TTX-sensitive Na+ current in a dose dependent manner (EC50 = 326.8 +/- 34.5 nM). Voltage and time dependence of the Na+ current were described in terms of the Hodgkin-Huxley model and compared in control conditions and in the presence of 500 nM BmK M1. The BmK M1 shifted steady state inactivation by 10.8 mV to less negative potentials. The steady state activation was shifted by 5.5 mV to more negative potentials, making the activation window larger. Moreover, BmK M1 increased the fast time constant of inactivation, leaving the activation time constant unchanged. In summary, BmK M1 primarily affected the inactivation parameters of the voltage gated Na+ current in isolated locust DUM neurons. (C) 2003 Elsevier Science Ltd. All rights reserved.
Notes: Limburgs Univ Ctr, Physiol Lab, B-3590 Diepenbeek, Belgium. Univ Leuven, Toxicol Lab, B-3000 Louvain, Belgium. Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China.Van Kerkhove, E, Limburgs Univ Ctr, Physiol Lab, Univ Campus Gebouw D, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/2451
DOI: 10.1016/S0022-1910(02)00263-9
ISI #: 000181679100008
ISSN: 0022-1910
Category: A1
Type: Journal Contribution
Validation: ecoom, 2004
Appears in Collections: Research publications

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