Document Server@UHasselt >
Research >
Research publications >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/24462

Title: Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure
Authors: Winckelmans, Ellen
Nawrot, Tim S.
Tsamou, Maria
Den Hond, Elly
Baeyens, Willy
Kleinjans, Jos
Lefebvre, Wouter
Van Larebeke, Nicolas
Peusens, Martien
Plusquin, Michelle
Reynders, Hans
Schoeters, Greet
Vanpoucke, Charlotte
de Kok, Theo M.
Vrijens, Karen
Issue Date: 2017
Citation: ENVIRONMENTAL HEALTH, 16, p. 1-15 (Art N° 87)
Abstract: Background: Due to their lack of repair capacity mitochondria are critical targets for environmental toxicants. We studied genes and pathways reflecting mitochondrial responses to short-and medium-term PM10 exposure. Methods: Whole genome gene expression was measured in peripheral blood of 98 adults (49% women). We performed linear regression analyses stratified by sex and adjusted for individual and temporal characteristics to investigate alterations in gene expression induced by short-term (week before blood sampling) and medium-term (month before blood sampling) PM10 exposure. Overrepresentation analyses (ConsensusPathDB) were performed to identify enriched mitochondrial associated pathways and gene ontology sets. Thirteen Human MitoCarta genes were measured by means of quantitative real-time polymerase chain reaction (qPCR) along with mitochondrial DNA (mtDNA) content in an independent validation cohort (n = 169, 55.6% women). Results: Overrepresentation analyses revealed significant pathways (p-value <0.05) related to mitochondrial genome maintenance and apoptosis for short-term exposure and to the electron transport chain (ETC) for medium-term exposure in women. For men, medium-term PM10 exposure was associated with the Tri Carbonic Acid cycle. In an independent study population, we validated several ETC genes, including UQCRH and COX7C (q-value <0.05), and some genes crucial for the maintenance of the mitochondrial genome, including LONP1 (q-value: 0.07) and POLG (q-value: 0.04) in women. Conclusions: In this exploratory study, we identified mitochondrial genes and pathways associated with particulate air pollution indicating upregulation of energy producing pathways as a potential mechanism to compensate for PM-induced mitochondrial damage.
Notes: [Winckelmans, Ellen; Nawrot, Tim S.; Tsamou, Maria; Peusens, Martien; Plusquin, Michelle; Vrijens, Karen] Hasselt Univ, Ctr Environm Sci, Agoralaan Gebouw D, B-3590 Diepenbeek, Belgium. [Nawrot, Tim S.] Leuven Univ, Dept Publ Hlth & Primary Care, Leuven, Belgium. [Den Hond, Elly] Prov Inst Hyg, Antwerp, Belgium. [Baeyens, Willy] Vrije Univ Brussel, Dept Analyt & Environm Chem, Brussels, Belgium. [Kleinjans, Jos; de Kok, Theo M.] Maastricht Univ, Dept Toxicogen, Maastricht, Netherlands. [Lefebvre, Wouter; Schoeters, Greet] Flemish Inst Technol Res, Mol, Belgium. [Van Larebeke, Nicolas] Univ Ghent, Dept Radiotherapy & Nucl Med, Ghent, Belgium. [Reynders, Hans] Flemish Govt, Nat & Energy Dept, Environm, Brussels, Belgium. [Schoeters, Greet] Univ Antwerp, Dept Biomed Sci, Antwerp, Belgium. [Schoeters, Greet] Univ Southern Denmark, Dept Environm Med, Inst Publ Hlth, Odense, Denmark. [Vanpoucke, Charlotte] Belgian Interreg Environm Agcy IRCEL, Brussels, Belgium.
URI: http://hdl.handle.net/1942/24462
DOI: 10.1186/s12940-017-0292-7
ISI #: 000408044200001
ISSN: 1476-069X
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

Files in This Item:

Description SizeFormat
Published version857.47 kBAdobe PDF

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.