Document Server@UHasselt >
Research >
Research publications >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/24442

Title: Valve interstitial cell culture: Production of mature type I collagen and precise detection
Authors: Liskova, Jana
Hadraba, Daniel
Filova, Elena
Konarik, Miroslav
Pirk, Jan
Jelen, Karel
Bacakova, Lucie
Issue Date: 2017
Publisher: WILEY
Citation: MICROSCOPY RESEARCH AND TECHNIQUE, 80(8), p. 936-942
Abstract: Collagen often acts as an extracellular and intracellular marker for in vitro experiments, and its quality defines tissue constructs. To validate collagen detection techniques, cardiac valve interstitial cells were isolated from pigs and cultured under two different conditions; with and without ascorbic acid. The culture with ascorbic acid reached higher cell growth and collagen deposition, although the expression levels of collagen gene stayed similar to the culture without ascorbic acid. The fluorescent microscopy was positive for collagen fibers in both the cultures. Visualization of only extracellular collagen returned a higher correlation coefficient when comparing the immunolabeling and second harmonic generation microscopy images in the culture with ascorbic acid. Lastly, it was proved that the hydroxyproline strongly contributes to the second-order susceptibility tensor of collagen molecules, and therefore the second harmonic generation signal is impaired in the culture without ascorbic acid.
Notes: [Liskova, Jana; Hadraba, Daniel; Filova, Elena; Bacakova, Lucie] Czech Acad Sci, Inst Physiol, Prague 14220, Czech Republic. [Hadraba, Daniel; Jelen, Karel] Charles Univ Prague, Fac Phys Educ & Sport, Prague 16200, Czech Republic. [Hadraba, Daniel] Hasselt Univ, Dept Biophys, B-3590 Diepenbeek, Belgium. [Konarik, Miroslav; Pirk, Jan] Inst Clin & Expt Med, Prague 14021, Czech Republic.
URI: http://hdl.handle.net/1942/24442
DOI: 10.1002/jemt.22886
ISI #: 000406242500015
ISSN: 1059-910X
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

Files in This Item:

Description SizeFormat
Published version959.63 kBAdobe PDF

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.