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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/24371

Title: Identification, evolution and functional characterization of two Zn CDF-family transporters of the ectomycorrhizal fungus Suillus luteus
Authors: Ruytinx, Joske
Coninx, Laura
Nguyen, Hoai
Smisdom, Nick
Morin, Emmanuelle
Kohler, Annegret
Cuypers, Ann
Colpaert, Jan V.
Issue Date: 2017
Publisher: WILEY
Abstract: Two genes, SlZnT1 and SlZnT2, encoding Cation Diffusion Facilitator (CDF) family transporters were isolated from Suillus luteus mycelium by genome walking. Both gene models are very similar and phylogenetic analysis indicates that they are most likely the result of a recent gene duplication event. Comparative sequence analysis of the deduced proteins predicts them to be Zn transporters. This function was confirmed by functional analysis in yeast for SlZnT1. SlZnT1 was able to restore growth of the highly Zn sensitive yeast mutant zrc1 and localized to the vacuolar membrane. Transformation of zrc1 yeast cells with SlZnT1 resulted in an increased accumulation of Zn compared to empty vector transformed zrc1 yeast cells and equals Zn accumulation in wild type yeast cells. We were not able to express functional SlZnT2 in yeast. In S. luteus, both SlZnT genes are constitutively expressed whatever the external Zn concentrations. A labile Zn pool was detected in the vacuoles of S. luteus free-living mycelium. Therefore we conclude that SlZnT1 is indispensable for maintenance of Zn homeostasis by transporting excess Zn into the vacuole.
Notes: [Ruytinx, Joske; Coninx, Laura; Nguyen, Hoai; Cuypers, Ann; Colpaert, Jan V.] Hasselt Univ, Ctr Environm Sci, Environm Biol, Agoralaan Bldg D, B-3590 Diepenbeek, Belgium. [Smisdom, Nick] Hasselt Univ, Biomed Res Inst, Agoralaan Bldg C, B-3590 Diepenbeek, Belgium. [Morin, Emmanuelle; Kohler, Annegret] Univ Lorraine Interact Arbres Microorganismes, Inst Natl Rech Agron, Lab Excellence ARBRE, UMR1136, F-54280 Champenoux, France.
URI: http://hdl.handle.net/1942/24371
DOI: 10.1111/1758-2229.12551
ISI #: 000405931900010
ISSN: 1758-2229
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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