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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/24370

Title: Genetic stability of ectomycorrhizal fungi is not affected by cryopreservation at-130 A degrees C or cold storage with repeated sub-cultivations over a period of 2 years
Authors: Crahay, Charlotte
Munaut, Francoise
Colpaert, Jan V.
Huret, Stephanie
Declerck, Stephane
Issue Date: 2017
Publisher: SPRINGER
Citation: MYCORRHIZA, 27(6), p. 595-601
Abstract: Cryopreservation is considered the most reliable method for storage of filamentous fungi including ectomycorrhizal (ECM) fungi. A number of studies, however, have reported genetic changes in fungus cultures following cryopreservation. In the present study, the genetic stability of six ECM fungus isolates was analyzed using amplified fragment length polymorphism (AFLP). The isolates were preserved for 2 years either by cryopreservation (at -130 A degrees C) or by storage at 4 A degrees C with regular sub-cultivation. A third preservation treatment consisting of isolates maintained on Petri dishes at 22-23 A degrees C for 2 years (i.e., without any sub-cultivation) was included and used as a control. The differences observed in AFLP patterns between the three preservation methods remained within the range of the total error generated by the AFLP procedure (6.85%). Therefore, cryopreservation at -130 A degrees C and cold storage with regular sub-cultivation did not affect the genetic stability of the ECM fungus isolates, and both methods can be used for the routine storage of ECM fungus isolates over a period of 2 years.
Notes: [Crahay, Charlotte; Declerck, Stephane] Catholic Univ Louvain, Appl Microbiol, Earth & Life Inst, Mycol, Croix Sud 2,Bte L7-05-06, B-1348 Louvain La Neuve, Belgium. [Munaut, Francoise] Catholic Univ Louvain, Appl Microbiol, Earth & Life Inst, Phytopathol, Croix Sud 2,Bte L7-05-03, B-1348 Louvain La Neuve, Belgium. [Colpaert, Jan V.] Univ Hasselt, Ctr Environm Sci, Environm Biol Grp, Agoralaan, B-3590 Diepenbeek, Belgium. [Huret, Stephanie] Catholic Univ Louvain, Earth & Life Inst, Appl Microbiol, Mycol,MUCL, Croix Sud 2,Box L7-05-06, B-1348 Louvain La Neuve, Belgium.
URI: http://hdl.handle.net/1942/24370
DOI: 10.1007/s00572-017-0770-3
ISI #: 000405925100007
ISSN: 0940-6360
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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