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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/24351

Title: A novel, highly conserved metallothionein family in basidiomycete fungi and characterization of two representative SlMTa and SlMTb genes in the ectomycorrhizal fungus Suillus luteus
Authors: Nguyen, Hoai
Rineau, Francois
Vangronsveld, Jaco
Cuypers, Ann
Colpaert, Jan V.
Ruytinx, Joske
Issue Date: 2017
Publisher: WILEY
Citation: ENVIRONMENTAL MICROBIOLOGY, 19(7), p. 2577-2587
Abstract: The basidiomycete Suillus luteus is an important member of the ectomycorrhizal community that thrives in heavy metal polluted soils covered with pioneer pine forests. This study aimed to identify potential heavy metal chelators in S. luteus. Two metallothionein (MT) coding genes, SlMTa and SlMTb, were identified. When heterologously expressed in yeast, both SlMTa and SlMTb can rescue the Cu sensitive mutant from Cu toxicity. In S. luteus, transcription of both SlMTa and SlMTb is induced by Cu but not Cd or Zn. Several putative Cu-sensing and metal-response elements are present in the promoter sequences. These results indicate that SlMTa and SlMTb function as Cu-thioneins. Homologs of the S. luteus MTs are present in 49 species belonging to 10 different orders of the subphylum Agaricomycotina and are remarkably conserved. The length of the proteins, number and distribution of cysteine residues indicate a novel family of fungal MTs. The ubiquitous and highly conserved features of these MTs suggest that they are important for basic cellular functions in species in the subphylum Agaricomycotina.
Notes: [Hoai Nguyen; Rineau, Francois; Vangronsveld, Jaco; Cuypers, Ann; Colpaert, Jan V.; Ruytinx, Joske] Hasselt Univ, Ctr Environm Sci, Environm Biol, Agoralaan Bldg D, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/24351
DOI: 10.1111/1462-2920.13729
ISI #: 000405907300007
ISSN: 1462-2912
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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