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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/24300

Title: Direct detection of nano-scale extracellular vesicles derived from inflammation-triggered endothelial cells using surface plasmon resonance
Authors: Hosseinkhani, Baharak
van den Akker, Nynke
D'Haen, Jan
Gagliardi, Mick
Struys, Tom
Lambrichts, Ivo
Waltenberger, Johannes
Nelissen, Inge
Hooyberghs, Jef
Molin, Daniel G. M.
Michiels, Luc
Issue Date: 2017
Abstract: A major conceptual breakthrough in cell signaling has been the finding of EV as new biomarker shuttles in body fluids. Now, one of the major challenges in using these nanometer-sized biological entities as diagnostic marker is the development of translational methodologies to profile them. SPR offers a promising label-free and real time platform with a high potential for biomarker detection. Therefore, we aimed to develop a uniform SPR methodology to detect specific surface markers on EV derived from patient with CHD. EVs having an approximate size range between 30 and 100 nm (similar to 48.5%) and 100-300 nm (similar to 51.5%) were successfully isolated. The biomarker profile of EV was verified using immunogold labeling, ELISA and SPR. Using SPR, we demonstrated an increased binding of EV derived from patients with CHD to anti-ICAM-1 antibodies as compared to EV from healthy donors. Our current findings open up novel opportunities for in-depth and label-free investigation of EV. (C) 2017 The Authors. Published by Elsevier Inc.
Notes: [Hosseinkhani, Baharak; Struys, Tom; Lambrichts, Ivo; Michiels, Luc] Hasselt Univ, Biomed Res Inst, Martelarenlaan 42, B-3500 Hasselt, Belgium. [D'Haen, Jan] Hasselt Univ, Inst Mat Res, Hasselt, Belgium. [Hooyberghs, Jef] Hasselt Univ, Theoret Phys, Hasselt, Belgium. [van den Akker, Nynke; Gagliardi, Mick; Molin, Daniel G. M.] Maastricht Univ, Dept Physiol, Cardiovasc Res Inst Maastricht CARIM, Maastricht, Netherlands. [Waltenberger, Johannes] Univ Hosp Munster, Dept Cardiovasc Med, Munster, Germany. [Waltenberger, Johannes] Univ Munster, Cells In Mot Cluster Excellence EXC CiM 1003, Munster, Germany. [Hooyberghs, Jef] Flemish Inst Technol Res VITO, Mol, Belgium.
URI: http://hdl.handle.net/1942/24300
DOI: 10.1016/j.nano.2017.03.010
ISI #: 000403662500009
ISSN: 1549-9634
Category: A1
Type: Journal Contribution
Validation: ecoom, 2018
Appears in Collections: Research publications

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