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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/24250

Title: Phthalate-induced oxidative stress and association with asthma-related airway inflammation in adolescents
Authors: Franken, Carmen
Lambrechts, Nathalie
Govarts, Eva
Koppen, Gudrun
Den Hond, Elly
Ooms, Daniella
Voorspoels, Stefan
Bruckers, Liesbeth
Loots, Ilse
Nelen, Vera
Sioen, Isabelle
Nawrot, Tim S.
Baeyens, Willy
Van Larebeke, Nicolas
Schoeters, Greet
Issue Date: 2017
Abstract: Background: In Belgium, around 8.5% of the children have asthmatic symptoms. Increased asthma risk in children has been reported in relation to exposure to phthalate plasticizers but the underlying mechanisms are largely unknown. Aim: The aim of this study was to identify if oxidative stress, assessed by excision of 8-hydroxydeoxyguanosine (8-OHdG) from damaged DNA, is an intermediate marker for the association between phthalate exposure and doctor-diagnosed asthma. Material and methods: In 41814-15-year-old youngsters, recruited as a representative sample of residents of Flanders (Belgium), personal exposure to phthalates was assessed by measuring phthalate metabolites in urine: mono(2-ethylhexyl) phthalate (MEHP), mono(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP), mono(2-ethyl-5-oxohexyl) phthalate (MEOHP), mono-n-butyl phthalate (MnBP), mono-benzyl phthalate (MBzP), mono-isobutyl phthalate (MiBP) and mono-ethyl phthalate (MEP). Analysis of 8-OHdG in urine was used as a sensitive biomarker of oxidative stress at the level of DNA. The presence of doctor diagnosed asthma was elicited by a self-administered questionnaire. Associations were assessed using multiple linear and logistic regression models. Mediation was tested using Baron and Kenny's regression approach. Results: A significant increased risk of a youngster being diagnosed with asthma was found for both urinary MnBP (metabolite of dibutyl phthalate (DBP)) and the sum of the three di(2-ethylhexyl) phthalate metabolites (Sigma DEHP = MEHP + MEHHP + MEOHP), with respective odds ratio of 1.84 [95% CI: 1.02,3.32] for MnBP and 1.94 [95% CI: 1.07, 3.51] for Sigma DEHP.In addition, we observed significant associations between all urinary phthalate metabolites and increased urinary levels of 8-OHdG. The associations were stronger in girls than in boys. We did not found evidence that 8-OHdG was associated with doctor-diagnosed asthma. Conclusion: The results of our study are in line with other findings from epidemiological surveys and raise further concern about DEHP and DBP as risk factors for asthma, however, the underlying mechanisms are not yet well understood. (C) 2017 Elsevier GmbH. All rights reserved.
Notes: [Franken, Carmen; Lambrechts, Nathalie; Govarts, Eva; Koppen, Gudrun; Ooms, Daniella; Voorspoels, Stefan; Schoeters, Greet] Flemish Inst Technol Res VITO, Boeretang 200, B-2400 Mol, Belgium. [Franken, Carmen; Schoeters, Greet] Univ Antwerp, Dept Biomed Sci, Antwerp, Belgium. [Den Hond, Elly; Nelen, Vera] Prov Inst Hyg, Antwerp, Belgium. [Bruckers, Liesbeth] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, Hasselt, Belgium. [Loots, Ilse] Univ Antwerp, Polit & Social Sci, Antwerp, Belgium. [Sioen, Isabelle] Univ Ghent, Dept Publ Hlth, Ghent, Belgium. [Sioen, Isabelle] Univ Ghent, Dept Food Safety & Food Qual, Ghent, Belgium. [Nawrot, Tim S.] Hasselt Univ, Ctr Environm Sci, Diepenbeek, Belgium. [Nawrot, Tim S.] Leuven Univ, Dept Publ Hlth & Primary Care, Leuven, Belgium. [Baeyens, Willy; Van Larebeke, Nicolas] Vrije Univ Brussel, Dept Analyt & Environm Chem, Brussels, Belgium. [Van Larebeke, Nicolas] Univ Ghent, Dept Radiotherapy & Nucl Med, Ghent, Belgium. [Schoeters, Greet] Univ Southern Denmark, Inst Publ Hlth, Dept Environm Med, Odense, Denmark.
URI: http://hdl.handle.net/1942/24250
DOI: 10.1016/j.ijheh.2017.01.006
ISI #: 000401215300018
ISSN: 1438-4639
Category: A1
Type: Journal Contribution
Validation: ecoom, 2018
Appears in Collections: Research publications

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