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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/24176

Title: Characterization and genomic analyses of two newly isolated Morganella phages define distant members among Tevenvirinae and Autographivirinae subfamilies
Authors: Oliveira, Hugo
Pinto, Graca
Oliveira, Ana
Noben, Jean-Paul
Hendrix, Hanne
Lavigne, Rob
Lobocka, Malgorzata
Kropinski, Andrew M.
Azeredo, Joana
Issue Date: 2017
Citation: SCIENTIFIC REPORTS, 7, p. 1-14 (Art N° 46157)
Abstract: Morganella morganii is a common but frequent neglected environmental opportunistic pathogen which can cause deadly nosocomial infections. The increased number of multidrug-resistant M. morganii isolates motivates the search for alternative and effective antibacterials. We have isolated two novel obligatorily lytic M. morganii bacteriophages (vB_ MmoM_ MP1, vB_ MmoP_ MP2) and characterized them with respect to specificity, morphology, genome organization and phylogenetic relationships. MP1' s dsDNA genome consists of 163,095 bp and encodes 271 proteins, exhibiting low DNA (< 40%) and protein (< 70%) homology to other members of the Tevenvirinae. Its unique property is a > 10 kb chromosomal inversion that encompass the baseplate assembly and head outer capsid synthesis genes when compared to other T- even bacteriophages. MP2 has a dsDNA molecule with 39,394 bp and encodes 55 proteins, presenting significant genomic (70%) and proteomic identity (86%) but only to Morganella bacteriophage MmP1. MP1 and MP2 are then novel members of Tevenvirinae and Autographivirinae, respectively, but differ significantly from other tailed bacteriophages of these subfamilies to warrant proposing new genera. Both bacteriophages together could propagate in 23 of 27 M. morganii clinical isolates of different origin and antibiotic resistance profiles, making them suitable for further studies on a development of bacteriophage cocktail for potential therapeutic applications.
Notes: [Oliveira, Hugo; Pinto, Graca; Oliveira, Ana; Azeredo, Joana] Univ Minho, CEB Ctr Biol Engn, LIBRO, P-4710057 Braga, Portugal. [Noben, Jean-Paul] Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium. [Noben, Jean-Paul] Hasselt Univ, Transnatl Univ Limburg, B-3590 Diepenbeek, Belgium. [Hendrix, Hanne; Lavigne, Rob] Katholieke Univ Leuven, Lab Gene Technol, Kasteelpk Arenberg 21 Box 2462, B-3001 Leuven, Belgium. [Lobocka, Malgorzata] Polish Acad Sci, Inst Biochem & Biophys, Dept Microbial Biochem, Warsaw, Poland. [Lobocka, Malgorzata] Warsaw Univ Life Sci, Fac Agr & Biol, Autonomous Dept Microbial Biol, Warsaw, Poland. [Kropinski, Andrew M.] Univ Guelph, Dept Food Sci, Guelph, ON N1G 2W1, Canada. [Kropinski, Andrew M.] Univ Guelph, Dept Mol & Cellular Biol, Guelph, ON N1G 2W1, Canada. [Kropinski, Andrew M.] Univ Guelph, Dept Pathobiol, Guelph, ON N1G 2W1, Canada.
URI: http://hdl.handle.net/1942/24176
DOI: 10.1038/srep46157
ISI #: 000398637600001
ISSN: 2045-2322
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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