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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/24118

Title: Didehydrophenylalanine, an abundant modification in the beta subunit of plant polygalacturonases
Authors: Sergeant, Kjell
Printz, Bruno
Gutsch, Annelie
Behr, Marc
Renaut, Jenny
Hausman, Jean-Francois
Issue Date: 2017
Publisher: PUBLIC LIBRARY SCIENCE
Citation: PLOS ONE, 12(2) (Art N° e0171990)
Abstract: The structure and the activity of proteins are often regulated by transient or stable post-translational modifications (PTM). Different from well-known, abundant modifications such as phosphorylation and glycosylation some modifications are limited to one or a few proteins across a broad range of related species. Although few examples of the latter type are known, the evolutionary conservation of these modifications and the enzymes responsible for their synthesis suggest an important physiological role. Here, the first observation of a new, fold-directing PTM is described. During the analysis of alfalfa cell wall proteins a -2Da mass shift was observed on phenylalanine residues in the repeated tetrapeptide FxxY of the beta-subunit of polygalacturonase. This modular protein is known to be involved in developmental and stress-responsive processes. The presence of this modification was confirmed using in-house and external datasets acquired by different commonly used techniques in proteome studies. Based on these analyses it was found that all identified phenylalanine residues in the sequence FxxY of this protein were modified to a, alpha,beta-didehydro-Phe (Delta Phe). Besides showing the reproducible identification of Delta Phe in different species arguments that substantiate the fold-determining role of Delta Phe are given.
Notes: [Sergeant, Kjell; Printz, Bruno; Gutsch, Annelie; Behr, Marc; Renaut, Jenny; Hausman, Jean-Francois] LIST, Environm Res & Innovat ERIN Dept, Esch Sur Alzette, Luxembourg. [Printz, Bruno; Behr, Marc] Catholic Univ Louvain, Earth & Life Inst Agron, Grp Rech Physiol Vegetale Louvain la Neuve, Louvain La Neuve, Belgium. [Gutsch, Annelie] Univ Hasselt, Ctr Environm Sci, Environm Biol, Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/24118
DOI: 10.1371/journal.pone.0171990
ISI #: 000394424500068
ISSN: 1932-6203
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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