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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/24104

Title: Cd-induced Cu deficiency responses in Arabidopsis thaliana: are phytochelatins involved?
Authors: Gielen, Heidi
Vangronsveld, Jaco
Cuypers, Ann
Issue Date: 2017
Publisher: WILEY
Citation: PLANT CELL AND ENVIRONMENT, 40(3), p. 390-400
Abstract: Cadmium (Cd) exposure can disturb the homeostasis of essential elements. In Arabidopsis thaliana, Cd induces a squamosa promoter binding protein-like 7 (SPL7)-dependent Cu deficiency response. We investigated how Cd induces a Cu deficiency response. The Cu deficiency response consists of the active SPL7 transcription factor binding to GTAC motifs in promoters of among others several Cu transporters, a Cu chaperone, and cupro-miRNAs to regulate Cu homeostasis. We demonstrated that the addition of supplemental Cu to Cd-exposed A.thaliana plants diminished the Cu deficiency response in roots, while it even disappeared in leaves. Exposure of plants to Cd in combination with extra Cu reduced Cd levels in both roots and leaves resulting in an improved cellular oxidative state. Furthermore, we demonstrated a role for phytochelatins (PCs) in the Cd-induced Cu deficiency response, because it was reduced in roots of cad1-3 mutant plants exposed to Cd. In conclusion, a working mechanism is provided in which it is suggested that Cd increases PC levels that can complex both Cd and Cu. This results in cellular Cu deficiency and subsequently the activation of SPL7 and hence the induction of the Cu deficiency response. Summary statement We demonstrated that addition of supplemental Cu to Cd-exposed Arabidopsis thaliana plants diminished the SPL7-dependent Cu deficiency response in roots, while it even disappeared in leaves. Moreover, in roots of phytochelatin (PC-) deficient cad1-3 mutants, this Cu deficiency response was reduced. Therefore, a working mechanism is provided in which it is suggested that Cd increases PC levels that can complex both Cd and Cu. This results in cellular Cu deficiency and subsequently the activation of SPL7 and hence the induction of the Cu deficiency response.
Notes: [Gielen, Heidi; Vangronsveld, Jaco; Cuypers, Ann] Hasselt Univ, Ctr Environm Sci, Environm Biol, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/24104
DOI: 10.1111/pce.12876
ISI #: 000395094000007
ISSN: 0140-7791
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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