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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/24024

Title: Dietary and supplemental maternal methyl-group donor intake and cord blood DNA methylation
Authors: Pauwels, Sara
Ghosh, Manosij
Duca, Radu Corneliu
Bekaert, Bram
Freson, Kathleen
Huybrechts, Inge
Langie, Sabine A. S.
Koppen, Gudrun
Devlieger, Roland
Godderis, Lode
Issue Date: 2017
Citation: EPIGENETICS, 12(1), p. 1-10
Status: In Press
Abstract: Maternal nutrition is critically involved in the development and health of the fetus. We evaluated maternal methyl-group donor intake through diet (methionine, betaine, choline, folate) and supplementation (folic acid) before and during pregnancy in relation to global DNA methylation and hydroxymethylation and gene specific (IGF2 DMR, DNMT1, LEP, RXRA) cord blood methylation. A total of 115 mother-infant pairs were enrolled in the MAternal Nutrition and Offspring's Epigenome (MANOE) study. The intake of methyl-group donors was assessed using a food-frequency questionnaire. LC-MS/MS and pyrosequencing were used to measure global and gene specific methylation, respectively. Dietary intake of methyl-groups before and during pregnancy was associated with changes in LEP, DNMT1, and RXRA cord blood methylation. Statistically significant higher cord blood LEP methylation was observed when mothers started folic acid supplementation more than 6 months before conception compared with 3-6 months before conception (34.6 +/- 6.3% vs. 30.1 +/- 3.6%, P = 0.011, LEP CpG1) or no folic acid used before conception (16.2 +/- 4.4% vs. 13.9 +/- 3%, P = 0.036 for LEP CpG3 and 24.5 +/- 3.5% vs. 22.2 +/- 3.5%, P = 0.045 for LEP mean CpG). Taking folic acid supplements during the entire pregnancy resulted in statistically significantly higher cord blood RXRA methylation as compared with stopping supplementation in the second trimester (12.3 +/- 1.9% vs. 11.1 +/- 2%, P = 0.008 for RXRA mean CpG). To conclude, long-term folic acid use before and during pregnancy was associated with higher LEP and RXRA cord blood methylation, respectively. To date, pregnant women are advised to take a folic acid supplement of 400 mu g/day from 4 weeks before until 12 weeks of pregnancy. Our results suggest significant epigenetic modifications when taking a folic acid supplement beyond the current advice.
Notes: [Pauwels, Sara; Ghosh, Manosij; Duca, Radu Corneliu; Godderis, Lode] KU Leuven Univ Leuven, Dept Publ Hlth & Primary Care, Environm & Hlth, Leuven, Belgium. [Pauwels, Sara; Langie, Sabine A. S.; Koppen, Gudrun] Flemish Inst Technol Res VITO, Unit Environm Risk & Hlth, Mol, Belgium. [Bekaert, Bram] KU Leuven Univ Leuven, Dept Imaging & Pathol, Leuven, Belgium. [Bekaert, Bram] KU Leuven Univ Leuven, Univ Hosp Leuven, Dept Forens Med, Lab Forens Genet & Mol Archeol, Leuven, Belgium. [Freson, Kathleen] KU Leuven Univ Leuven, Ctr Mol & Vasc Biol, Leuven, Belgium. [Huybrechts, Inge] Int Agcy Res Canc, Dietary Exposure Assessment Grp, Lyon, France. [Langie, Sabine A. S.] Hasselt Univ, Fac Sci, Diepenbeek, Belgium. [Devlieger, Roland] KU Leuven Univ Leuven, Dept Dev & Regenerat, Leuven, Belgium. [Devlieger, Roland] Univ Hosp Leuven, Dept Obstet & Gynecol, Leuven, Belgium. [Godderis, Lode] IDEWE, External Serv Prevent & Protect, Heverlee, Belgium.
URI: http://hdl.handle.net/1942/24024
DOI: 10.1080/15592294.2016.1257450
ISI #: 000394449200001
ISSN: 1559-2294
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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