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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/24003

Title: The plant pathogen Erwinia amylovora: a proteome investigation
Authors: Holtappels, Michelle
Noben, Jean-Paul
Valcke, Roland
Vrancken, Kristof
Schoofs, Hilde
Deckers, Tom
Issue Date: 2015
Publisher: International Society for Horticultural Science
Citation: Hale, C.; Hunter, D.; Roberts, W.; Ikin, R.; McMaugh, S. (Ed.). XXIX INTERNATIONAL HORTICULTURAL CONGRESS ON HORTICULTURE: SUSTAINING LIVES, LIVELIHOODS AND LANDSCAPES (IHC2014): INTERNATIONAL SYMPOSIA ON INNOVATIVE PLANT PROTECTION IN HORTICULTURE, BIOSECURITY, QUARANTINE PESTS, AND MARKET ACCESS, International Society for Horticultural Science,p. 235-240
Series/Report: Acta Horticulturae
Abstract: Erwinia amylovora is considered to be a homogeneous species although different strains show differences in virulence. In this investigation, two wild type strains with differences in virulence, a high virulent strain PFB5 and a low virulent strain LMG2024, were compared using a proteomic approach. Hereby, proteins were identified which were important for this difference in virulence between both strains. Bacteria were grown in a minimal medium and at mid-exponential phase, samples were taken. The complete proteome was extracted and a 2D differential in-gel electrophoresis (DIGE) approach was used. Differently abundant spots were excised, trypsinized and identified. Eventually 31 spots were identified as being important in the difference between the two strains. Flagellin (FliC) and a chemotaxis regulatory protein (CheY) were more abundant in the low virulent strain. A protein important in amylovoran synthesis, UTP-glucose-1-phosphate uridylyltransferase (GalF), was upregulated in the high virulent strain. Afterwards, phenotypic experiments were performed to validate the proteomic results.
URI: http://hdl.handle.net/1942/24003
DOI: 10.17660/ActaHortic.2015.1105.33
ISI #: 000378738600033
ISBN: 9789462610996
ISSN: 0567-7572
Category: C1
Type: Proceedings Paper
Validation: ecoom, 2017
Appears in Collections: Research publications

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